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product-image-AAA18802_IHC7.png IHC (Immunohistochemistry) (IHC image of AAA18802 diluted at1:500 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigenretrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Thenprimary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualizedusing an HRP conjugated SP system.)

Rabbit anti-Human, Mouse IgG receptor FcRn large subunit p51 Polyclonal Antibody | anti-IgG antibody

Rabbit anti-human IgG receptor FcRn large subunit p51 polyclonal Antibody

Gene Names
FCGRT; FCRN; alpha-chain
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
IgG receptor FcRn large subunit p51, Antibody; Rabbit anti-human IgG receptor FcRn large subunit p51 polyclonal Antibody; IgG Fc fragment receptor transporter alpha chain FCGRT FCRN; anti-IgG antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Sequence Length
365
Applicable Applications for anti-IgG antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:20-1:1000
IF: 1:50-1:200
Conjugate
Non-conjugated
Immunogen
Recombinant human IgG receptor FcRn large subunit p51 protein (24-297AA)
Immunogen Species
Homo sapiens (Human)
Research Area
Immunology
Target Names
FCGRT
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

IHC (Immunohistochemistry)

(IHC image of AAA18802 diluted at1:500 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigenretrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Thenprimary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualizedusing an HRP conjugated SP system.)

product-image-AAA18802_IHC7.png IHC (Immunohistochemistry) (IHC image of AAA18802 diluted at1:500 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigenretrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Thenprimary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualizedusing an HRP conjugated SP system.)

IHC (Immunohistchemistry)

(IHC image of AAA18802 diluted at1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigenretrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Thenprimary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

product-image-AAA18802_IHC6.png IHC (Immunohistchemistry) (IHC image of AAA18802 diluted at1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigenretrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Thenprimary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IF (Immunofluorescence)

(Immunofluorescent analysis of HepG2 cells using AAA18802 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

product-image-AAA18802_IF5.jpg IF (Immunofluorescence) (Immunofluorescent analysis of HepG2 cells using AAA18802 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human Pancreatic Cancer using AAA18802 at dilution 1:100)

product-image-AAA18802_IHC4.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human Pancreatic Cancer using AAA18802 at dilution 1:100)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human placenta tissue using AAA18802 at dilution 1:100)

product-image-AAA18802_IHC3.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human placenta tissue using AAA18802 at dilution 1:100)

WB (Western Blot)

(Western blotAll lanes: FCGRT antibody at 1:1000Lane 1: Mouse liver tissueLane 2: Mouse kidney tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 40 kDaObserved band size: 40 kDa)

product-image-AAA18802_WB2.jpg WB (Western Blot) (Western blotAll lanes: FCGRT antibody at 1:1000Lane 1: Mouse liver tissueLane 2: Mouse kidney tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 40 kDaObserved band size: 40 kDa)

WB (Western Blot)

(All lanes: FCGRT antibody at 1:2000Lane 1: HepG2 whole cell lysateLane 2: Mouse liver tissueLane 3: A549 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 40 kDaObserved band size: 40 kDa)

product-image-AAA18802_WB.png WB (Western Blot) (All lanes: FCGRT antibody at 1:2000Lane 1: HepG2 whole cell lysateLane 2: Mouse liver tissueLane 3: A549 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 40 kDaObserved band size: 40 kDa)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
IgG receptor FcRn large subunit p51
NCBI Official Synonym Full Names
Fc fragment of IgG receptor and transporter
NCBI Official Symbol
FCGRT
NCBI Official Synonym Symbols
FCRN; alpha-chain
NCBI Protein Information
IgG receptor FcRn large subunit p51
UniProt Protein Name
IgG receptor FcRn large subunit p51
UniProt Gene Name
FCGRT
UniProt Synonym Gene Names
FCRN; FcRn
UniProt Entry Name
FCGRN_HUMAN

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Product Notes

The IgG fcgrt (Catalog #AAA18802) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human IgG receptor FcRn large subunit p51 polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IgG receptor FcRn large subunit p51 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:20-1:1000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the IgG fcgrt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG receptor FcRn large subunit p51, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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