Rabbit IK Polyclonal Antibody | anti-IK antibody

IK Polyclonal Antibody

Cat. Num. AAA268815

• Gene Names: IK; RED; RER; CSA2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: IK, Antibody; IK Polyclonal Antibody; anti-IK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IK Polyclonal Antibody detects endogenous levels of IK protein
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 557

• Applicable Applications for anti-IK antibody: ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: The antiserum was produced against synthesized peptide derived from human RED. AA range:508-557.
• Function: caution: Was originally (PubMed:7970704) thought to be the IK factor, a cytokine involved in the negative regulatory pathway of constitutive MHC class II antigens expression developmental stage: Expressed at similar levels in fetal and adult tissues.,function: Not known. May bind to chromatin.,PTM: Phosphorylated upon DNA damage, probably by ATM or ATR,similarity: Belongs to the RED family.,tissue specificity: Ubiquitous.
• Subcellular Location: Nucleus. Nucleus, nucleoplasm . Chromosome . Cytoplasm, cytoskeleton, spindle pole . Predominantly present throughout the nucleoplasm during prometaphase, metaphase and anaphase. Is also detected in nuclear foci that are not identical with Cajal bodies. Starts to accumulate at chromosomes during telophase, and is nearly exclusively associated with chromosomes in newly divided cells (PubMed:24252166). Colocalizes with MAD1L1 at mitotic spindle poles during metaphase and anaphase (PubMed:22351768).
• Expression: Ubiquitous.
• Preparation and Storage: -15 degree to -25 degree /1 year (Do not lower than -25 degree.

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4 degree overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).)

WB (Western Blot)

(Western blot analysis of lysates from HepG2, MCF-7, COLO, and HeLa cells, using RED antibody. The lane on the right is blocked with the synthesized peptide.)

Related Product Information for anti-IK antibody

The protein encoded by this gene was identified by its RED repeat, a stretch of repeated arginine, glutamic acid and aspartic acid residues. The protein localizes to discrete dots within the nucleus, excluding the nucleolus. Its function is unknown. This gene maps to chromosome 5; however, a pseudogene may exist on chromosome 2. [provided by RefSeq, Jul 2008].

NCBI and Uniprot Product Information

• NCBI GI #: 125988409
• NCBI GeneID: 3550
• NCBI Accession #: NP_006074.2
• NCBI GenBank Nucleotide #: NP_006074.2
• UniProt Accession #: Q13123
• Molecular Weight: 65kDa
• NCBI Official Full Name: protein Red
• NCBI Official Synonym Full Names: IK cytokine
• NCBI Official Symbol: IK
• NCBI Official Synonym Symbols: RED; RER; CSA2
• NCBI Protein Information: protein Red
• UniProt Protein Name: Protein Red
• UniProt Gene Name: IK
• UniProt Synonym Gene Names: RED; RER
• UniProt Entry Name: RED_HUMAN