IHC (Immunohiostchemistry)
(Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH 6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (IL-13) Polyclonal Antibody at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.)
Rabbit IL-13 Polyclonal Antibody | anti-IL-13 antibody
IL-13
Reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Sheep
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Protein A affinity purified.
Synonyms
IL-13, Antibody; IL-13; ALRH; BHR 1; BHR1; IL 13; IL13; Interleukin 13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30; P600; IL13_MOUSE; Interleukin-13; T-cell activation protein P600; anti-IL-13 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Sheep
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A affinity purified.
Form/Format
0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Applicable Applications for anti-IL-13 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
KLH conjugated synthetic peptide derived from mouse IL-13 45-56/131 (mo)
Positive control
Lymph node (Mouse) Lysate, Jurkat (Human) Cell Lysate, Molt-4 (Human) Cell Lysate, Human brain glioma.
Subcellular location
Secreted
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze/thaw cycles.
IHC (Immunohiostchemistry)
(Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH 6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (IL-13) Polyclonal Antibody at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.)
IHC (Immunohiostchemistry)
(Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH 6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (IL-13) Polyclonal Antibody at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.)
WB (Western Blot)
(Sample: Lymph node (Mouse) Lysate at 40 ug. Primary: Anti-IL-13 at 1/500 dilution. Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 12 kD. Observed band size: 32 kD.)
WB (Western Blot)
(Sample: Lymph node (Mouse) Lysate at 40 ug. Primary: Anti-IL-13 at 1/500 dilution. Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 12 kD. Observed band size: 32 kD.)
Related Product Information for anti-IL-13 antibody
Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 was first cloned in 1993 and is located on chromosome 5q31 with a length of 1.4kb. It has a mass of 13 kDa and folds into 4 alpha helical bundles. The secondary structural features of IL-13 are similar to that of Interleukin 4 (IL-4); however it only has 25% sequence homology to IL4 and is capable of IL-4 independent signaling. Interleukin-13 is a central regulator in IgE synthesis, goblet cell hyperplasia, mucus hypersecretion, airway hyperresponsiveness, fibrosis and chitinase upregulation. It is a mediator of allergic inflammation and different diseases including asthma.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
12 KDa
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Product Notes
The IL-13 (Catalog #AAA269000) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL-13 reacts with Human, Mouse, Rat, Dog, Pig, Cow, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's IL-13 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the IL-13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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