IHC (Immunohiostchemistry)
(AAA323459 at 1/100 staining Human uterus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse IL12B Polyclonal Antibody | anti-IL12B antibody
IL12B Antibody
Gene Names
IL12B; CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
IL12B, Antibody; IL12B Antibody; CLMF; CLMF p40; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL 12 subunit p40; IL 12B; IL-12 subunit p40; IL-12B; IL12 subunit p40; IL12B; IL12B_HUMAN; interleukin 12 beta chain; Interleukin 12 p40; Interleukin 12 subunit beta; Interleukin 12B; Interleukin-12 subunit beta; natural killer cell stimulatory factor 40 kD subunit; NK cell stimulatory factor chain 2; NKSF; NKSF2; p40; anti-IL12B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
IL12B antibody detects endogenous levels of total IL12B
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
328
Applicable Applications for anti-IL12B antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Secreted. Secreted upon association with IL12B.
Conjugation
Unconjugated
IHC (Immunohiostchemistry)
(AAA323459 at 1/100 staining Human uterus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA323459 at 1/100 staining Human uterus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of IL12B expression in Human fetal liver lysate;, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of IL12B expression in Human fetal liver lysate;, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-IL12B antibody
Function: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Subunit Structure: Heterodimer with IL12A; disulfide-linked. The heterodimer is known as interleukin IL-12. Heterodimer with IL23A; disulfide-linked. The heterodimer is known as interleukin IL-23. Also secreted as a monomer.
Post-translational Modifications: Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.
Similarity: Belongs to the type I cytokine receptor family. Type 3 subfamily.
Subunit Structure: Heterodimer with IL12A; disulfide-linked. The heterodimer is known as interleukin IL-12. Heterodimer with IL23A; disulfide-linked. The heterodimer is known as interleukin IL-23. Also secreted as a monomer.
Post-translational Modifications: Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.
Similarity: Belongs to the type I cytokine receptor family. Type 3 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 46 kDa
Predicted: 38 kDa
Predicted: 38 kDa
NCBI Official Full Name
interleukin-12 subunit beta
NCBI Official Synonym Full Names
interleukin 12B
NCBI Official Symbol
IL12B
NCBI Official Synonym Symbols
CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B
NCBI Protein Information
interleukin-12 subunit beta
UniProt Protein Name
Interleukin-12 subunit beta
UniProt Gene Name
IL12B
UniProt Synonym Gene Names
NKSF2; IL-12B; CLMF p40; NKSF2
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Product Notes
The IL12B il12b (Catalog #AAA323459) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL12B Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL12B can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the IL12B il12b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL12B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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