WB (Western Blot)
(Anti-IL-18 Picoband antibody, AAA45630-2.jpgAll lanes: Anti-IL18 (AAA45630) at 0.5ug/mlWB: HELA Whole Cell Lysate at 40ugPredicted bind size: 22KDObserved bind size: 22KD)
Rabbit anti-Human IL18 Polyclonal Antibody | anti-IL18 antibody
Anti-IL18 antibody
Gene Names
IL18; IGIF; IL-18; IL-1g; IL1F4
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
IL18, Antibody; Anti-IL18 antibody; Interleukin-18; interleukin 18 (interferon-gamma-inducing factor); Iboctadekin antibody; IFN gamma inducing factor antibody; IFN-gamma-inducing factor antibody; IGIF antibody; IL 1 gamma antibody; IL 18 antibody; IL 1g antibody; IL-1 gamma antibody; IL-18 antibody; IL1 gamma antibody; IL18 antibody; IL18 protein antibody; IL1F4 antibody; IL1g antibody; IL1gamma antibody; ILIF4 antibody; Interferon gamma inducing factor antibody; Interferon gamma-inducing factor antibody; Interleukin 1 gamma antibody; Interleukin 18 antibody; Interleukin-1 gamma antibody; Interleukin-18 antibody; Interleukin18 antibody; MGC12320 antibody; anti-IL18 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
193
Applicable Applications for anti-IL18 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived human IL18 recombinant protein (Position: Y37-D193).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-IL-18 Picoband antibody, AAA45630-2.jpgAll lanes: Anti-IL18 (AAA45630) at 0.5ug/mlWB: HELA Whole Cell Lysate at 40ugPredicted bind size: 22KDObserved bind size: 22KD)
WB (Western Blot)
(Anti-IL-18 Picoband antibody, AAA45630-2.jpgAll lanes: Anti-IL18 (AAA45630) at 0.5ug/mlWB: HELA Whole Cell Lysate at 40ugPredicted bind size: 22KDObserved bind size: 22KD)
IHC (Immunohistochemistry)
(Anti-IL-18 Picoband antibody, AAA45630-1.JPGIHC(P): Human Tonsil Tissue)
IHC (Immunohistochemistry)
(Anti-IL-18 Picoband antibody, AAA45630-1.JPGIHC(P): Human Tonsil Tissue)
Related Product Information for anti-IL18 antibody
Description: Rabbit IgG polyclonal antibody for Interleukin-18(IL18) detection. Tested with WB, IHC-P in Human.
Background: Interleukin-18 also known as IL18 is a protein which in humans is encoded by the IL18 gene. The protein encoded by this gene is a proinflammatory cytokine.IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-gamma (IFN-gamma) or type II interferon that plays an important role in activating the macrophages or other cells.The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. The human interleukin 18 gene IL18 maps to 11q22.2-q22.3, closely linked to the DRD2 gene locus and distinct from mapped IDDM loci.
Background: Interleukin-18 also known as IL18 is a protein which in humans is encoded by the IL18 gene. The protein encoded by this gene is a proinflammatory cytokine.IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-gamma (IFN-gamma) or type II interferon that plays an important role in activating the macrophages or other cells.The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. The human interleukin 18 gene IL18 maps to 11q22.2-q22.3, closely linked to the DRD2 gene locus and distinct from mapped IDDM loci.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,896 Da
NCBI Official Full Name
Interleukin-18
NCBI Official Synonym Full Names
interleukin 18
NCBI Official Symbol
IL18
NCBI Official Synonym Symbols
IGIF; IL-18; IL-1g; IL1F4
NCBI Protein Information
interleukin-18; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)
UniProt Protein Name
Interleukin-18
UniProt Gene Name
IL18
UniProt Synonym Gene Names
IGIF; IL1F4; IL-18; IFN-gamma-inducing factor; IL-1 gamma
UniProt Entry Name
IL18_HUMAN
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Product Notes
The IL18 il18 (Catalog #AAA45630) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL18 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL18 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the IL18 il18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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