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product-image-AAA326806_WB11.jpg WB (Western Blot) (Western blot analysis of extracts of A549, using IL18R1antibody. The lane on the left is treated with the antigen-specific peptide.)

Rabbit IL18R1 Polyclonal Antibody | anti-IL18R1 antibody

IL18R1 Antibody

Gene Names
IL18R1; CD218a; IL18RA; IL1RRP; CDw218a; IL-1Rrp
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific)
Synonyms
IL18R1, Antibody; IL18R1 Antibody; CD218 antigen-like family member A; CD218a; CDw218a; CDw218a antigen; IL 1Rrp; IL-18R-1; IL-18R1; IL-1Rrp; IL1 receptor related protein; IL1 receptor-related protein; IL18R_HUMAN; IL18R1; IL18RA; Il18ralpha; IL1R-rp; IL1RRP; Interleukin 18 receptor 1; Interleukin 18 receptor, alpha chain; Interleukin-18 receptor 1; anti-IL18R1 antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific)
Form/Format
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-IL18R1 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human IL18R1,corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

WB (Western Blot)

(Western blot analysis of extracts of A549, using IL18R1antibody. The lane on the left is treated with the antigen-specific peptide.)

product-image-AAA326806_WB11.jpg WB (Western Blot) (Western blot analysis of extracts of A549, using IL18R1antibody. The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence)

(AAA326806 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

product-image-AAA326806_IF13.jpg IF (Immunofluorescence) (AAA326806 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry)

(AAA326806 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

product-image-AAA326806_IHC15.jpg IHC (Immunohistochemistry) (AAA326806 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-IL18R1 antibody
The protein encoded by this gene is a cytokine receptor thatbelongs to the interleukin 1 receptor family. This receptorspecifically binds interleukin 18 (IL18), and is essential forIL18 mediated signal transduction. IFN-alpha and IL12 arereported to induce the expression of this receptor in NK andT cells. This gene along with four other members of theinterleukin 1 receptor family, including IL1R2, IL1R1, ILRL2(IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster onchromosome 2q. [provided by RefSeq, Jul 2008

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
62kDa
NCBI Official Full Name
interleukin-18 receptor 1 isoform 2
NCBI Official Synonym Full Names
interleukin 18 receptor 1
NCBI Official Symbol
IL18R1
NCBI Official Synonym Symbols
CD218a; IL18RA; IL1RRP; CDw218a; IL-1Rrp
NCBI Protein Information
interleukin-18 receptor 1
UniProt Protein Name
Interleukin-18 receptor 1
UniProt Gene Name
IL18R1
UniProt Synonym Gene Names
IL-18R-alpha; IL-18Ralpha4 PublicationsManual assertion based on opinion iniRef.6

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Product Notes

The IL18R1 il18r1 (Catalog #AAA326806) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL18R1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IL18R1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the IL18R1 il18r1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL18R1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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