WB (Western Blot)
(Anti-IL-7 Receptor alpha chain pY449 - Western Blot. Anti-IL-7 Receptor alpha chain pY449 in immunoprecipitation and Western blot. Lysates were prepared from thymocyte D1 cells treated with or without IL7 (50 ng/ml), and immunoprecipitated with anti-Phosphotyrosine conjugated to Protein G agarose. After washing and boiling, the supernatant was separated by SDS-PAGE and transferred to nitrocellulose. Western Blot analysis was performed using anti-IL7 receptor pY449, diluted 1:10000 in BSA, incubated overnight at 4°C. The proteins were visualized using an HRP-conjugated goat anti-rabbit antibody and ECL detection.)
Rabbit IL7R / CD127 Polyclonal Antibody | anti-IL7R antibody
Rabbit Polyclonal to Mouse IL7R / CD127
Gene Names
IL7R; ILRA; CD127; IL7RA; CDW127; IL-7R-alpha
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
IL7R / CD127, Antibody; Rabbit Polyclonal to Mouse IL7R / CD127; Anti-IL7R / CD127 Antibody (phospho-Tyr449) IHC-plus; IL7R; CD127; CD127 antigen; IL-7RA; IL-7 receptor subunit alpha; IL7RA; ILRA; Interleukin 7 receptor; CDW127; IL-7R subunit alpha; IL-7R-alpha; Mouse IL7R; anti-IL7R antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
A BLAST analysis was used to suggest cross-reactivity with IL-7R from human, mouse and rat sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-7R from other sources has not been determined.
Purity/Purification
Immunoaffinity Purified
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
459
Applicable Applications for anti-IL7R antibody
ELISA, WB (Western Blot), IHC (Immunohistochemistry)
Target Species
Mouse
Immunogen Description
Synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.
Immunogen Type
Synthetic peptide
Immunogen
IL7R / CD127 antibody was raised against synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.
Antigen Modification
pTyr449
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-IL-7 Receptor alpha chain pY449 - Western Blot. Anti-IL-7 Receptor alpha chain pY449 in immunoprecipitation and Western blot. Lysates were prepared from thymocyte D1 cells treated with or without IL7 (50 ng/ml), and immunoprecipitated with anti-Phosphotyrosine conjugated to Protein G agarose. After washing and boiling, the supernatant was separated by SDS-PAGE and transferred to nitrocellulose. Western Blot analysis was performed using anti-IL7 receptor pY449, diluted 1:10000 in BSA, incubated overnight at 4°C. The proteins were visualized using an HRP-conjugated goat anti-rabbit antibody and ECL detection.)
WB (Western Blot)
(Anti-IL-7 Receptor alpha chain pY449 - Western Blot. Anti-IL-7 Receptor alpha chain pY449 in immunoprecipitation and Western blot. Lysates were prepared from thymocyte D1 cells treated with or without IL7 (50 ng/ml), and immunoprecipitated with anti-Phosphotyrosine conjugated to Protein G agarose. After washing and boiling, the supernatant was separated by SDS-PAGE and transferred to nitrocellulose. Western Blot analysis was performed using anti-IL7 receptor pY449, diluted 1:10000 in BSA, incubated overnight at 4°C. The proteins were visualized using an HRP-conjugated goat anti-rabbit antibody and ECL detection.)
IHC (Immunohistochemistry)
(Anti-IL-7 Receptor antibody IHC of human colon, MALT. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-IL-7 Receptor antibody IHC of human colon, MALT. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Related Product Information for anti-IL7R antibody
Interleukin 7 Receptor (IL7R) is a receptor for interleukin 7 (IL7). The function of IL7R requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in the V(D)J recombination during lymphocyte development. This protein is also found to control the accessibility of the TCR gamma locus by STAT5 and histone acetylation.
Product Categories/Family for anti-IL7R antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,724 Da
NCBI Official Full Name
interleukin-7 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 7 receptor
NCBI Official Symbol
IL7R
NCBI Official Synonym Symbols
ILRA; CD127; IL7RA; CDW127; IL-7R-alpha
NCBI Protein Information
interleukin-7 receptor subunit alpha; IL-7RA; CD127 antigen; IL-7R subunit alpha; IL-7 receptor subunit alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6
UniProt Protein Name
Interleukin-7 receptor subunit alpha
UniProt Gene Name
IL7R
UniProt Synonym Gene Names
IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA
UniProt Entry Name
IL7RA_HUMAN
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Product Notes
The IL7R il7r (Catalog #AAA50907) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Mouse IL7R / CD127 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IL7R / CD127 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the IL7R il7r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL7R / CD127, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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