WB (Western Blot)
(Western blot analysis of ILK (arrow) using rabbit polyclonal ILK Antibody (pS246). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ILK gene.)
Rabbit ILK Polyclonal Antibody | anti-ILK antibody
ILK Antibody (S246)
Gene Names
ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ILK, Antibody; ILK Antibody (S246); Integrin-linked protein kinase; 59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK; ILK; ILK1; ILK2; anti-ILK antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ILK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-253 amino acids from human ILK.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.43 (varies by lot)
Sequence Positions
225-253
Sequence Length
452
Applicable Applications for anti-ILK antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of ILK (arrow) using rabbit polyclonal ILK Antibody (pS246). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ILK gene.)
WB (Western Blot)
(Western blot analysis of ILK (arrow) using rabbit polyclonal ILK Antibody (pS246). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ILK gene.)
WB (Western Blot)
(Western blot analysis of anti-ILK Antibody (S246) in Hela cell line lysates (35ug/lane). ILK(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-ILK Antibody (S246) in Hela cell line lysates (35ug/lane). ILK(arrow) was detected using the purified Pab.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ILK Antibody (S246) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ILK Antibody (S246) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Related Product Information for anti-ILK antibody
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. ILK is a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction.
Product Categories/Family for anti-ILK antibody
References
Li, Y., et al., J. Clin. Invest. 112(4):503-516 (2003).
Troussard, A.A., et al., J. Biol. Chem. 278(25):22374-22378 (2003).
Marotta, A., et al., Br. J. Cancer 88(11):1755-1762 (2003).
Cordes, N., et al., Br. J. Cancer 88(9):1470-1479 (2003).
Fukuda, T., et al., J. Cell Biol. 160(7):1001-1008 (2003).
Troussard, A.A., et al., J. Biol. Chem. 278(25):22374-22378 (2003).
Marotta, A., et al., Br. J. Cancer 88(11):1755-1762 (2003).
Cordes, N., et al., Br. J. Cancer 88(9):1470-1479 (2003).
Fukuda, T., et al., J. Cell Biol. 160(7):1001-1008 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51419
NCBI Official Full Name
integrin-linked protein kinase isoform 1
NCBI Official Synonym Full Names
integrin-linked kinase
NCBI Official Symbol
ILK
NCBI Official Synonym Symbols
P59; ILK-1; ILK-2; p59ILK; HEL-S-28
NCBI Protein Information
integrin-linked protein kinase
UniProt Protein Name
Integrin-linked protein kinase
UniProt Gene Name
ILK
UniProt Synonym Gene Names
ILK1; ILK2
UniProt Entry Name
ILK_HUMAN
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Product Notes
The ILK ilk (Catalog #AAA286051) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 225-253. The ILK Antibody (S246) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's ILK can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the ILK ilk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ILK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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