WB (Western Blot)
(Detection of human and mouse Importin 11/IPO11 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Importin 11/IPO11 antibody AAA212952 (lot AAA212952-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse Importin 11/IPO11 Polyclonal Antibody | anti-IPO11 antibody
Rabbit anti-Importin 11/IPO11 Antibody, Affinity Purified
Gene Names
IPO11; RanBP11
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Importin 11/IPO11, Antibody; Rabbit anti-Importin 11/IPO11 Antibody, Affinity Purified; RanBP11; imp11; importin-11; Ran binding protein 11; ran-binding protein 11; Importin-11; importin 11; anti-IPO11 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-IPO11 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 925 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse Importin 11/IPO11 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Importin 11/IPO11 antibody AAA212952 (lot AAA212952-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse Importin 11/IPO11 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Importin 11/IPO11 antibody AAA212952 (lot AAA212952-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human Importin 11/IPO11 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Importin 11/IPO11 antibody AAA212952 (lot AAA212952-1) used for IP at 6 ug per reaction. Importin 11/IPO11 was also immunoprecipitated by rabbit anti-Importin 11/IPO11 antibody For blotting immunoprecipitated Importin 11/IPO11, AAA212952 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human Importin 11/IPO11 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Importin 11/IPO11 antibody AAA212952 (lot AAA212952-1) used for IP at 6 ug per reaction. Importin 11/IPO11 was also immunoprecipitated by rabbit anti-Importin 11/IPO11 antibody For blotting immunoprecipitated Importin 11/IPO11, AAA212952 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-IPO11 antibody
Importin 11 functions in nuclear protein import as nuclear transport receptor. It serves as receptor for nuclear localization signals (NLS) in cargo substrates, and is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Importin 11/IPO11 mediates the nuclear import of UBE2E3, and of RPL12 (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9UI26].
Product Categories/Family for anti-IPO11 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
112,535 Da
NCBI Official Full Name
importin-11 isoform 2
NCBI Official Synonym Full Names
importin 11
NCBI Official Symbol
IPO11
NCBI Official Synonym Symbols
RanBP11
NCBI Protein Information
importin-11; imp11; Ran binding protein 11; ran-binding protein 11
UniProt Protein Name
Importin-11
UniProt Gene Name
IPO11
UniProt Synonym Gene Names
RANBP11; Imp11; RanBP11
UniProt Entry Name
IPO11_HUMAN
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Product Notes
The IPO11 ipo11 (Catalog #AAA212952) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Importin 11/IPO11 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Importin 11/IPO11 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the IPO11 ipo11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Importin 11/IPO11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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