WB (Western Blot)
(Detection of human and mouse Importin 7 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Importin 7 antibody AAA211983 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 10 ug/mg lysate. Importin 7 was also immunoprecipitated by rabbit anti-Importin 7 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).)
Rabbit anti-Human, Mouse Importin 7 Polyclonal Antibody | anti-IPO7 antibody
Rabbit anti-Importin 7 Antibody, Affinity Purified
Gene Names
IPO7; Imp7; RANBP7
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Importin 7, Antibody; Rabbit anti-Importin 7 Antibody, Affinity Purified; Imp7; RANBP7; importin-7; RAN-binding protein 7; Importin-7; importin 7; anti-IPO7 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-IPO7 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 225 and 275
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse Importin 7 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Importin 7 antibody AAA211983 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 10 ug/mg lysate. Importin 7 was also immunoprecipitated by rabbit anti-Importin 7 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).)
WB (Western Blot)
(Detection of human and mouse Importin 7 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Importin 7 antibody AAA211983 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 10 ug/mg lysate. Importin 7 was also immunoprecipitated by rabbit anti-Importin 7 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).)
IHC (Immunohistochemistry)
(Detection of human Importin 7 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Importin 7 (Cat. No. AAA211983) used at a dilution of 1:1,000 (0.2ug/ml) Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Importin 7 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Importin 7 (Cat. No. AAA211983) used at a dilution of 1:1,000 (0.2ug/ml) Detection: DAB)
Related Product Information for anti-IPO7 antibody
Importin 7 autonomously mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. It binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with KPNB1 it mediates the nuclear import of H1 histone. In vitro, importin 7 mediates nuclear import of H2A, H2B, H3 and H4 histones. Also, in vitro, it mediates the nuclear import of HIV-1 reverse transcription complex (RTC) integrase and HIF-1 alpha [taken from the Universal Protein Resource (UniProt) ww.uniprot.org/uniprot/O95373].
Product Categories/Family for anti-IPO7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
119,517 Da
NCBI Official Full Name
importin-7
NCBI Official Synonym Full Names
importin 7
NCBI Official Symbol
IPO7
NCBI Official Synonym Symbols
Imp7; RANBP7
NCBI Protein Information
importin-7; RAN-binding protein 7
UniProt Protein Name
Importin-7
UniProt Gene Name
IPO7
UniProt Synonym Gene Names
RANBP7; Imp7; RanBP7
UniProt Entry Name
IPO7_HUMAN
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Product Notes
The IPO7 ipo7 (Catalog #AAA211983) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Importin 7 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Importin 7 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the IPO7 ipo7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Importin 7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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