WB (Western Blot)
(Detection of human INO80 by western blot. Samples: Whole cell lysate (15 ug) from Jurkat, A-549, Hep-G2, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INO80 antibody AAA212156 (lot AAA212156-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human INO80 Polyclonal Antibody | anti-INO80 antibody
Rabbit anti-INO80 Antibody, Affinity Purified
Gene Names
INO80; INOC1; INO80A; hINO80
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
INO80, Antibody; Rabbit anti-INO80 Antibody, Affinity Purified; putative DNA helicase INO80 complex homolog 1; chromatin-remodeling ATPase INO80; DNA helicase INO80; DNA helicase-related INO80 complex homolog 1; DNA helicase-related protein INO80; hINO80; homolog of yeast INO80; INO80 complex subunit A; INO80A; INOC1; INO80 complex subunit; anti-INO80 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-INO80 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1506 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human INO80 by western blot. Samples: Whole cell lysate (15 ug) from Jurkat, A-549, Hep-G2, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INO80 antibody AAA212156 (lot AAA212156-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human INO80 by western blot. Samples: Whole cell lysate (15 ug) from Jurkat, A-549, Hep-G2, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INO80 antibody AAA212156 (lot AAA212156-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human INO80 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INO80 antibody AAA212156 (lot AAA212156-2) used for IP at 6 ug per reaction. INO80 was also immunoprecipitated by a previous lot of this antibody (lot AAA212156-1) and rabbit anti-INO80 antibody For blotting immunoprecipitated INO80, AAA212156 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human INO80 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INO80 antibody AAA212156 (lot AAA212156-2) used for IP at 6 ug per reaction. INO80 was also immunoprecipitated by a previous lot of this antibody (lot AAA212156-1) and rabbit anti-INO80 antibody For blotting immunoprecipitated INO80, AAA212156 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-INO80 antibody
DNA helicase INO80 (INO80) is a DNA helicase and probable main scaffold component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9ULG1].
Product Categories/Family for anti-INO80 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
176,753 Da
NCBI Official Full Name
DNA helicase INO80
NCBI Official Synonym Full Names
INO80 complex subunit
NCBI Official Symbol
INO80
NCBI Official Synonym Symbols
INOC1; INO80A; hINO80
NCBI Protein Information
DNA helicase INO80; INO80 homolog; homolog of yeast INO80; INO80 complex subunit A; putative DNA helicase INO80 complex homolog 1
UniProt Protein Name
DNA helicase INO80
UniProt Gene Name
INO80
UniProt Synonym Gene Names
INO80A; INOC1; KIAA1259; hINO80
UniProt Entry Name
INO80_HUMAN
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Product Notes
The INO80 ino80 (Catalog #AAA212156) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-INO80 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's INO80 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the INO80 ino80 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "INO80, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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