ICC (Immunocytochemistry)
(Immunocytochemistry of INPP4B in 3T3 cells with INPP4B antibody at 2.5 μg/ml.)
Rabbit INPP4B Polyclonal Antibody | anti-INPP4B antibody
INPP4B Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot, ELISA
Purity
Affinity chromatography purified via peptide column.
Synonyms
INPP4B, Antibody; INPP4B Antibody; Inositol polyphosphate-4-phosphatase type II 105kDa; anti-INPP4B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
INPP4B antibody is human, mouse and rat reactive. At least two isoforms of INPP4B are known to exist; this antibody only detects the longer isoform. INPP4B antibody is predicted to not cross-react with INPP4A.
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
924
Applicable Applications for anti-INPP4B antibody
ICC (Immunocytochemistry), WB (Western Blot), ELISA
Immunogen
INPP4B antibody was raised against a 17 amino acid peptide near the amino terminus of human INPP4B.
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
3T3 Cell Lysate
Conjugate
Unconjugated
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
ICC (Immunocytochemistry)
(Immunocytochemistry of INPP4B in 3T3 cells with INPP4B antibody at 2.5 μg/ml.)
ICC (Immunocytochemistry)
(Immunocytochemistry of INPP4B in 3T3 cells with INPP4B antibody at 2.5 μg/ml.)
WB (Western Blot)
(Western blot analysis of INPP4B in 3T3 cell lysate with INPP4B antibody at 1 μg/ml.)
WB (Western Blot)
(Western blot analysis of INPP4B in 3T3 cell lysate with INPP4B antibody at 1 μg/ml.)
Related Product Information for anti-INPP4B antibody
Inositol polyphosphate 4-phosphatase type II (INPP4B) is one of the enzymes involved in phosphatidylinositol signaling pathways. It removes the phosphate group at position 4 of the inositol ring from inositol 3,4-bisphosphate (1). INPP4B can function as a tumor suppressor by inhibiting PI3K signaling. In human epithelial cells, knockdown of INPP4B expression resulted in enhanced Akt activation, anchorage-independent growth, and enhanced overall motility (2). It is thought INPP4B may also have oncogenic functions as INPP4B-dependent PI3K signaling is mediated by SGK3 in breast cancer cells and expression of INPP4B leads to enhanced SGK3 activation and suppression of AKT phosphorylation (3).
Product Categories/Family for anti-INPP4B antibody
References
Norris FA, Auethavekiat V, and Majerus PW. The isolation and characterization of cDNA encoding human and rat brain inositol polyphosphate 4-phosphase. J. Biol. Chem. 1995; 270:16128-33. Gewinner C, Wang ZC, Richardson A, et al. Evidence that inositol polyphosphate 4-phosphase type II is a tumor suppressor that inhibits PI3K signaling. Cancer Cell 2009; 16:115-25. Gasser JA, Inuzuka H, Lau AW, et al. SGK3 mediates INPP4B-dependent PI3K signaling in breast cancer. Mol. Cell 2014; 56:595-607.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 102 kDa
Observed: 100 kDa
Observed: 100 kDa
NCBI Official Full Name
type II inositol 3,4-bisphosphate 4-phosphatase
NCBI Official Synonym Full Names
inositol polyphosphate-4-phosphatase type II B
NCBI Official Symbol
INPP4B
NCBI Protein Information
type II inositol 3,4-bisphosphate 4-phosphatase
UniProt Protein Name
Type II inositol 3,4-bisphosphate 4-phosphatase
UniProt Gene Name
INPP4B
UniProt Entry Name
INP4B_HUMAN
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Product Notes
The INPP4B inpp4b (Catalog #AAA40841) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The INPP4B Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's INPP4B can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the INPP4B inpp4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "INPP4B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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