FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of Daudi cells using anti-INPP5F antibody (AAA127266).Overlay histogram showing Daudi cells stained with AAA127266 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-INPP5F Antibody (AAA127266, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit anti-Human INPP5F Polyclonal Antibody | anti-INPP5F antibody
Anti-INPP5F Antibody Picoband
Gene Names
INPP5F; SAC2; hSAC2; MSTP007; MSTPO47
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
INPP5F, Antibody; Anti-INPP5F Antibody Picoband; anti-INPP5F antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-INPP5F antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
E coli-derived human INPP5F recombinant protein (Position: H317-Q1112). Human INPP5F shares 87.6% amino acid (aa) sequence identity with mouse INPP5F.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of Daudi cells using anti-INPP5F antibody (AAA127266).Overlay histogram showing Daudi cells stained with AAA127266 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-INPP5F Antibody (AAA127266, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of Daudi cells using anti-INPP5F antibody (AAA127266).Overlay histogram showing Daudi cells stained with AAA127266 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-INPP5F Antibody (AAA127266, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of INPP5F using anti-INPP5F antibody (AAA127266).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human SH-SY5Y whole cell lysates,Lane 2: human SIHA whole cell lysates,Lane 3: human U251 whole cell lysates,Lane 4: human Caco-2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPP5F antigen affinity purified polyclonal antibody (#AAA127266) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for INPP5F at approximately 128 kDa. The expected band size for INPP5F is at 128 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of INPP5F using anti-INPP5F antibody (AAA127266).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human SH-SY5Y whole cell lysates,Lane 2: human SIHA whole cell lysates,Lane 3: human U251 whole cell lysates,Lane 4: human Caco-2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPP5F antigen affinity purified polyclonal antibody (#AAA127266) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for INPP5F at approximately 128 kDa. The expected band size for INPP5F is at 128 kDa.)
Related Product Information for anti-INPP5F antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-INPP5F antibody
References
1. Minagawa, T., Ijuin, T., Mochizuki, Y., Takenawa, T. Identification and characterization of a Sac domain-containing phosphoinositide 5-phosphatase. J. Biol. Chem. 276: 22011-22015, 2001. ?2. Nagase, T., Ishikawa, K., Suyama, M., Kikuno, R., Hirosawa, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 6: 63-70, 1999.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,800 Da
NCBI Official Full Name
phosphatidylinositide phosphatase SAC2 isoform 2
NCBI Official Synonym Full Names
inositol polyphosphate-5-phosphatase F
NCBI Official Symbol
INPP5F
NCBI Official Synonym Symbols
SAC2; hSAC2; MSTP007; MSTPO47
NCBI Protein Information
phosphatidylinositide phosphatase SAC2; Sac domain-containing inositol phosphatase 2; sac domain-containing phosphoinositide 5-phosphatase 2
UniProt Protein Name
Phosphatidylinositide phosphatase SAC2
UniProt Gene Name
INPP5F
UniProt Synonym Gene Names
KIAA0966; SAC2; hSAC2
UniProt Entry Name
SAC2_HUMAN
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Product Notes
The INPP5F inpp5f (Catalog #AAA127266) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-INPP5F Antibody Picoband reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's INPP5F can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the INPP5F inpp5f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "INPP5F, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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