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IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA230488 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Rabbit anti-Human Integrin-linked protein kinase Polyclonal Antibody | anti-ILK antibody
Rabbit anti-human Integrin-linked protein kinase polyclonal Antibody
Gene Names
ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Integrin-linked protein kinase, Antibody; Rabbit anti-human Integrin-linked protein kinase polyclonal Antibody; Integrin-linked protein kinase protein; 59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK; anti-ILK antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
452
Applicable Applications for anti-ILK antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human Integrin-linked protein kinase protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-7516 / sc-30713 / sc-13075
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA230488 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA230488 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human kidney using AAA230488 at dilution of 1:20)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human kidney using AAA230488 at dilution of 1:20)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded 293T cells using AAA230488 at dilution of 1:100 Immunohistochemistry)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded 293T cells using AAA230488 at dilution of 1:100 Immunohistochemistry)
WB (Western Blot)
(Western blotAll lanes: ILK antibody at 3ug/mlLane 1: Hela whole cell lysateLane 2: NIH/3T3 whole cell lysateLane 3: HL60 whole cell lysateLane 4: Mouse liver tissueLane 5: Mouse lung tissueLane 6: Rat liver tissueLane 7: Rat lung tissueLane 8: Zebrafish lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 52,45,37 kDaObserved band size: 51,37 kDa)
WB (Western Blot)
(Western blotAll lanes: ILK antibody at 3ug/mlLane 1: Hela whole cell lysateLane 2: NIH/3T3 whole cell lysateLane 3: HL60 whole cell lysateLane 4: Mouse liver tissueLane 5: Mouse lung tissueLane 6: Rat liver tissueLane 7: Rat lung tissueLane 8: Zebrafish lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 52,45,37 kDaObserved band size: 51,37 kDa)
Related Product Information for anti-ILK antibody
Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Product Categories/Family for anti-ILK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,467 Da
NCBI Official Full Name
integrin-linked protein kinase isoform 1
NCBI Official Synonym Full Names
integrin linked kinase
NCBI Official Symbol
ILK
NCBI Official Synonym Symbols
P59; ILK-1; ILK-2; p59ILK; HEL-S-28
NCBI Protein Information
integrin-linked protein kinase
UniProt Protein Name
Integrin-linked protein kinase
UniProt Gene Name
ILK
UniProt Synonym Gene Names
ILK1; ILK2
UniProt Entry Name
ILK_HUMAN
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Product Notes
The ILK ilk (Catalog #AAA230488) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Integrin-linked protein kinase polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin-linked protein kinase can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the ILK ilk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin-linked protein kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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