IF (Immunofluorescence)
(Fluorescent confocal image of HeLa cells stained with IFITM3 (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with Fragilis (IFITM3) (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Note the highly specific localization of the IFITM3 immunoreactivity to the Golgi.)
Rabbit anti-Human Interferon-inducible protein (IFITM3) Polyclonal Antibody | anti-IFITM3 antibody
Interferon-inducible protein (IFITM3) Antibody (N-term)
Gene Names
IFITM3; 1-8U; IP15; DSPA2b
Reactivity
Human
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Interferon-inducible protein (IFITM3), Antibody; Interferon-inducible protein (IFITM3) Antibody (N-term); Interferon-induced transmembrane protein 3; Dispanin subfamily A member 2b; DSPA2b; Interferon-inducible protein 1-8U; IFITM3; anti-IFITM3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Interferon-inducible protein (IFITM3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Interferon-inducible protein (IFITM3).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
1-30
Sequence Length
133
Applicable Applications for anti-IFITM3 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of HeLa cells stained with IFITM3 (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with Fragilis (IFITM3) (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Note the highly specific localization of the IFITM3 immunoreactivity to the Golgi.)
IF (Immunofluorescence)
(Fluorescent confocal image of HeLa cells stained with IFITM3 (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with Fragilis (IFITM3) (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Note the highly specific localization of the IFITM3 immunoreactivity to the Golgi.)
WB (Western Blot)
(IFITM3 Antibody (N-term) western blot analysis in Hela,293,A549,NCI-H460,HepG2,Sk-Br-3,A375 cell line lysates (35ug/lane).This demonstrates the IFITM3 antibody detected the IFITM3 protein (arrow).)
WB (Western Blot)
(IFITM3 Antibody (N-term) western blot analysis in Hela,293,A549,NCI-H460,HepG2,Sk-Br-3,A375 cell line lysates (35ug/lane).This demonstrates the IFITM3 antibody detected the IFITM3 protein (arrow).)
WB (Western Blot)
(Western blot analysis of lysate from HT1080 cell line,using IFITM3 Antibody (N-term). AAA283972 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysate from HT1080 cell line,using IFITM3 Antibody (N-term). AAA283972 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.)
Related Product Information for anti-IFITM3 antibody
The family of interferon-induced transmembrane protein (Ifitm/mil/fragilis) cell surface proteins may modulate cell adhesion and influence cell differentiation.
Product Categories/Family for anti-IFITM3 antibody
References
Tanaka,S.S., Dev. Cell 9 (6), 745-756 (2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14632
NCBI Official Full Name
interferon-induced transmembrane protein 3
NCBI Official Synonym Full Names
interferon induced transmembrane protein 3
NCBI Official Symbol
IFITM3
NCBI Official Synonym Symbols
1-8U; IP15; DSPA2b
NCBI Protein Information
interferon-induced transmembrane protein 3
UniProt Protein Name
Interferon-induced transmembrane protein 3
UniProt Gene Name
IFITM3
UniProt Synonym Gene Names
DSPA2b
UniProt Entry Name
IFM3_HUMAN
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Product Notes
The IFITM3 ifitm3 (Catalog #AAA283972) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The Interferon-inducible protein (IFITM3) Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Interferon-inducible protein (IFITM3) can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the IFITM3 ifitm3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interferon-inducible protein (IFITM3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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