WB (Western Blot)
(Detection of human IQGAP1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IQGAP1 antibody AAA211693 (lot AAA211693-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human IQGAP1 Polyclonal Antibody | anti-IQGAP1 antibody
Rabbit anti-IQGAP1 Antibody, Affinity Purified
Gene Names
IQGAP1; SAR1; p195; HUMORFA01
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
IQGAP1, Antibody; Rabbit anti-IQGAP1 Antibody, Affinity Purified; RasGAP-like with IQ motifs; HUMORFA01; SAR1; p195; ras GTPase-activating-like protein IQGAP1; Ras GTPase-activating-like protein IQGAP1; IQ motif containing GTPase activating protein 1; anti-IQGAP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-IQGAP1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human IQGAP1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IQGAP1 antibody AAA211693 (lot AAA211693-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human IQGAP1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IQGAP1 antibody AAA211693 (lot AAA211693-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human IQGAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IQGAP1 antibody AAA211693 (lot AAA211693-2) used for IP at 3 ug per reaction. IQGAP1 was also immunoprecipitated by a previous lot of this antibody (lot AAA211693-1). For blotting immunoprecipitated IQGAP1, AAA211693 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human IQGAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IQGAP1 antibody AAA211693 (lot AAA211693-2) used for IP at 3 ug per reaction. IQGAP1 was also immunoprecipitated by a previous lot of this antibody (lot AAA211693-1). For blotting immunoprecipitated IQGAP1, AAA211693 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human IQGAP1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-IQGAP1 (Cat. No. AAA211693 Lot2) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human IQGAP1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-IQGAP1 (Cat. No. AAA211693 Lot2) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-IQGAP1 antibody
IQGAP1 (IQ motif containing GTPase activating protein 1) interacts with the Rho-family GTPases, CDC42 and RAC1. It has been shown to localize to sites of cell-cell contact and regulate cadherin-mediated cell-cell adhesion. In addition to its role in cell adhesion, it is also involved in cell polarization and migration.
Product Categories/Family for anti-IQGAP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
189,252 Da
NCBI Official Full Name
ras GTPase-activating-like protein IQGAP1
NCBI Official Synonym Full Names
IQ motif containing GTPase activating protein 1
NCBI Official Symbol
IQGAP1
NCBI Official Synonym Symbols
SAR1; p195; HUMORFA01
NCBI Protein Information
ras GTPase-activating-like protein IQGAP1; RasGAP-like with IQ motifs
UniProt Protein Name
Ras GTPase-activating-like protein IQGAP1
UniProt Gene Name
IQGAP1
UniProt Synonym Gene Names
KIAA0051
UniProt Entry Name
IQGA1_HUMAN
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Product Notes
The IQGAP1 iqgap1 (Catalog #AAA211693) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-IQGAP1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IQGAP1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the IQGAP1 iqgap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IQGAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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