WB (Western Blot)
(Western blot analysis of IREB1 in lysates of HeLa, using IREB1 Antibody(AF4711).)
Rabbit IREB1 Polyclonal Antibody | anti-IREB1 antibody
IREB1 Antibody
Gene Names
ACO1; IRP1; ACONS; IREB1; IREBP; IREBP1
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (100%), Zebrafish (83%), Bovine (92%), Horse (92%), Sheep (92%), Rabbit (100%), Dog (92%), Chicken (92%), Xenopus (92%)
Applications
ELISA, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
IREB1, Antibody; IREB1 Antibody; ACO 1; ACO1; ACOC_HUMAN; Aconitase 1 soluble; Aconitase; Aconitase1; Aconitate hydratase; ACONS; Citrate hydro lyase; Citrate hydro-lyase; Cytoplasmic aconitate hydratase; Ferritin repressor protein; IRE BP 1; IRE-BP 1; IREB 1; IREB1; IREBP; IREBP1; Iron regulatory protein 1; Iron responsive element binding protein 1; Iron-responsive element-binding protein 1; IRP 1; IRP1; OTTHUMP00000045233; anti-IREB1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (83%), Bovine (92%), Horse (92%), Sheep (92%), Rabbit (100%), Dog (92%), Chicken (92%), Xenopus (92%)
Predicted Reactivity: Pig (100%), Zebrafish (83%), Bovine (92%), Horse (92%), Sheep (92%), Rabbit (100%), Dog (92%), Chicken (92%), Xenopus (92%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
IREB1 Antibody detects endogenous levels of total IREB1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-IREB1 antibody
ELISA (Peptide ELISA), WB (Western Blot)
Immunogen
A synthesized peptide derived from human IREB1, corresponding to a region within N-terminal amino acids.
Conjugation
Unconjugated
Fragment
Fab fragment
Subunit Structure
Interacts (when associated with the 4Fe-4S) with FBXL5. Interacts with frataxin (81-210).
Similarity
Belongs to the aconitase/IPM isomerase family.
Subcellular Location
Cytoplasm.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of IREB1 in lysates of HeLa, using IREB1 Antibody(AF4711).)
WB (Western Blot)
(Western blot analysis of IREB1 in lysates of HeLa, using IREB1 Antibody(AF4711).)
WB (Western Blot)
(Western blot analysis of extracts from Mouse brain, using IREB1 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Mouse brain, using IREB1 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-IREB1 antibody
Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
98,399 Da
NCBI Official Full Name
cytoplasmic aconitate hydratase
NCBI Official Synonym Full Names
aconitase 1, soluble
NCBI Official Symbol
ACO1
NCBI Official Synonym Symbols
IRP1; ACONS; IREB1; IREBP; IREBP1
NCBI Protein Information
cytoplasmic aconitate hydratase; IRE-BP 1; citrate hydro-lyase; iron regulatory protein 1; ferritin repressor protein; aconitate hydratase, cytoplasmic; iron-responsive element binding protein 1; iron-responsive element-binding protein 1
UniProt Protein Name
Cytoplasmic aconitate hydratase
UniProt Gene Name
ACO1
UniProt Synonym Gene Names
IREB1; Aconitase; IRP1; IRE-BP 1
UniProt Entry Name
ACOC_HUMAN
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Product Notes
The IREB1 aco1 (Catalog #AAA329534) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IREB1 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Zebrafish (83%), Bovine (92%), Horse (92%), Sheep (92%), Rabbit (100%), Dog (92%), Chicken (92%), Xenopus (92%) and may cross-react with other species as described in the data sheet. AAA Biotech's IREB1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), WB (Western Blot). Researchers should empirically determine the suitability of the IREB1 aco1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IREB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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