FCM/FACS (Flow Cytometry)
(IREB2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit IREB2 Polyclonal Antibody | anti-IREB2 antibody
IREB2 Antibody (Center)
Gene Names
IREB2; ACO3; IRP2; IRP2AD
Reactivity
Human (Predicted Reactivity: Mouse, Pig, Rat)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
IREB2, Antibody; IREB2 Antibody (Center); Iron-responsive element-binding protein 2; IRE-BP 2; Iron regulatory protein 2; IRP2; IREB2; anti-IREB2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Pig, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This IREB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 201-230 amino acids from the Central region of human IREB2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
201-230
Sequence Length
343
Applicable Applications for anti-IREB2 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Calculated MW
105045
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(IREB2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(IREB2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(IREB2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IREB2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(IREB2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IREB2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(IREB2 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IREB2 antibody detected the IREB2 protein (arrow).)
WB (Western Blot)
(IREB2 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IREB2 antibody detected the IREB2 protein (arrow).)
Related Product Information for anti-IREB2 antibody
IREB2 is a RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
Product Categories/Family for anti-IREB2 antibody
References
Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)
Hansen, H.M., et al. Hum. Mol. Genet. 19(18):3652-3661(2010)
Cho, M.H., et al. Nat. Genet. 42(3):200-202(2010)
Salahudeen, A.A., et al. Science 326(5953):722-726(2009)
DeMeo, D.L., et al. Am. J. Hum. Genet. 85(4):493-502(2009)
Hansen, H.M., et al. Hum. Mol. Genet. 19(18):3652-3661(2010)
Cho, M.H., et al. Nat. Genet. 42(3):200-202(2010)
Salahudeen, A.A., et al. Science 326(5953):722-726(2009)
DeMeo, D.L., et al. Am. J. Hum. Genet. 85(4):493-502(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
iron-responsive element-binding protein 2
NCBI Official Synonym Full Names
iron-responsive element binding protein 2
NCBI Official Symbol
IREB2
NCBI Official Synonym Symbols
ACO3; IRP2; IRP2AD
NCBI Protein Information
iron-responsive element-binding protein 2
UniProt Protein Name
Iron-responsive element-binding protein 2
UniProt Gene Name
IREB2
UniProt Synonym Gene Names
IRE-BP 2; IRP2
UniProt Entry Name
IREB2_HUMAN
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Product Notes
The IREB2 ireb2 (Catalog #AAA289676) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 201-230. The IREB2 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse, Pig, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's IREB2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the IREB2 ireb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IREB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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