IF (Immunofluorescence)
(Figure 2 Immunofluorescence Validation of IRGB10 in Mouse Spleen Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse spleen labeling IRGB10 with AAA40964 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Rabbit anti-Mouse IRGB10 Polyclonal Antibody | anti-IRGB10 antibody
IRGB10 (CT) Antibody
Reactivity
Mouse
Applications
Immunofluorescence, Western Blot, ELISA
Purity
IRGB10 Antibody is affinity chromatography purified via peptide column.
Synonyms
IRGB10, Antibody; IRGB10 (CT) Antibody; IRGB10 Antibody: Immunity-related GTPase family member b10; Interferon-gamma-inducible p47 GTPase; Gm12250; OTTMUSG00000005723; predicted gene 12250; anti-IRGB10 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
IRGB10 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid; IRGB10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-IRGB10 antibody
IF (Immunofluorescence), WB (Western Blot), ELISA
Immunogen
Anti-IRGB10 antibody (9515) was raised against a peptide corresponding to 13 amino acids near the C-terminus of mouse IRGB10.
The immunogen is located within the last 50 amino acids of IRGB10.
The immunogen is located within the last 50 amino acids of IRGB10.
Homology
Predicted species reactivity based on immunogen sequence: Monkey (100%), Gorilla (100%)
Research Area
Signal Transduction, Inflammasomes
NCBI Organism
Mus musculus
Isoforms
Mouse IRGB10 has 1 isoform (417aa, 47.2kD).
Preparation and Storage
IRGB10 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
IF (Immunofluorescence)
(Figure 2 Immunofluorescence Validation of IRGB10 in Mouse Spleen Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse spleen labeling IRGB10 with AAA40964 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 2 Immunofluorescence Validation of IRGB10 in Mouse Spleen Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse spleen labeling IRGB10 with AAA40964 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
WB (Western Blot)
(Figure 1 Western Blot Validation In Mouse Tissues Loading: 15ug of lysates per lane. Antibodies: IRGB10 AAA40964, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 1 Western Blot Validation In Mouse Tissues Loading: 15ug of lysates per lane. Antibodies: IRGB10 AAA40964, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
Related Product Information for anti-IRGB10 antibody
IRGB10 Antibody: IRGB10 is an inferno-inducible protein that is essential for the activation of DNA-sensing AIM2 inflammasome, and contributes to the activation of LPS-sensing caspase-11 and NLRP3 inflammasome. IRGB10 is part of a conserved signaling hub at the interface between cell-autonomous immunity and innate immune sensing pathways. IRGB10 targets cytoplasmic bacteria and compromises bacterial structural integrity. IRGB10 and LIGP2 act together to confer differential susceptibility against Chlamydia infection in mice.
Product Categories/Family for anti-IRGB10 antibody
References
Man et al. Cell 2016; 167(2):382-396.e17
Bernstein-Hanley I et al. Proc Natl Acad Sci USA. 2006;103(38):14092-14097
Miyairi I et al. J Immunol 2007; 179(3):1814-1824
Coers J et al. J Immunol 2008; 180(9):6237-6245
Bernstein-Hanley I et al. Proc Natl Acad Sci USA. 2006;103(38):14092-14097
Miyairi I et al. J Immunol 2007; 179(3):1814-1824
Coers J et al. J Immunol 2008; 180(9):6237-6245
NCBI and Uniprot Product Information
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Product Notes
The IRGB10 (Catalog #AAA40964) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IRGB10 (CT) Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IRGB10 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the IRGB10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRGB10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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