ICC (Immunocytochemistry)
(Immunocytochemistry of IRS-1 in P815 cells with IRS-1 antibody at 2 ug/mL.)
Rabbit anti-Human, Mouse IRS-1 Polyclonal Antibody | anti-IRS-1 antibody
IRS-1 Antibody
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Western Blot, ELISA
Purity
Affinity chromatography purified via peptide column
Synonyms
IRS-1, Antibody; IRS-1 Antibody; Insulin receptor substrate-1; anti-IRS-1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Affinity chromatography purified via peptide column
Form/Format
Supplied in PBS containing 0.02% sodium azide.
Sequence Length
1242
Applicable Applications for anti-IRS-1 antibody
ICC (Immunocytochemistry), WB (Western Blot), ELISA
Immunogen Type
Peptide
Immunogen Description
Raised against a 16 amino acid peptide from near the center of human IRS-1.
Target Name
IRS-1
Preparation and Storage
Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
ICC (Immunocytochemistry)
(Immunocytochemistry of IRS-1 in P815 cells with IRS-1 antibody at 2 ug/mL.)
ICC (Immunocytochemistry)
(Immunocytochemistry of IRS-1 in P815 cells with IRS-1 antibody at 2 ug/mL.)
WB (Western Blot)
(Western blot analysis of IRS-1 in PC-3 cell lysate with IRS-1 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.)
WB (Western Blot)
(Western blot analysis of IRS-1 in PC-3 cell lysate with IRS-1 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.)
Related Product Information for anti-IRS-1 antibody
Following tyrosine phosphorylation, the insulin receptor substrate 1 and 2 (IRS-1 and IRS-2) can form a protein scaffolding for the assembly of a host of Src homology 2 (SH2) domain-containing proteins. IRS-1 tyrosine phosphorylation can occur through the activity of several cytokine and growth factor receptors such as interleukin (IL)-4, IL-9, interferon-gamma, in addition to the insulin and insulin-like growth factor 1 receptors. The scaffolding provided by IRS-1 and IRS-2 is necessary for insulin signal transduction across the cell membrane. IRS-1 tyrosine phosphorylation, and thus formation of the IRS scaffolding is inhibited by tumor necrosis factor (TNF), and this inhibition can itself be blocked by rapamycin, an inhibitor of the mammalian target of rapamycin (TOR). TNF activity could also be blocked by inhibition of the Akt kinase and the PTEN tumor suppressor, suggesting that TNF impairs insulin signaling through IRS-1 by activation of the TOR signaling pathway.
Product Categories/Family for anti-IRS-1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
131,591 Da
NCBI Official Full Name
insulin receptor substrate 1
NCBI Official Synonym Full Names
insulin receptor substrate 1
NCBI Official Symbol
IRS1
NCBI Official Synonym Symbols
HIRS-1
NCBI Protein Information
insulin receptor substrate 1
UniProt Protein Name
Insulin receptor substrate 1
UniProt Gene Name
IRS1
UniProt Synonym Gene Names
IRS-1
UniProt Entry Name
IRS1_HUMAN
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Product Notes
The IRS-1 irs1 (Catalog #AAA306795) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IRS-1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IRS-1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the IRS-1 irs1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRS-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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