IHC (Immunohiostchemistry)
(Islet 1 was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- Islet 1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
Rabbit anti-Human, Rat Islet 1 Polyclonal Antibody | anti-ISL1 antibody
Anti-Islet 1 Antibody
Gene Names
ISL1; Isl-1; ISLET1
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Islet 1, Antibody; Anti-Islet 1 Antibody; ISL 1; ISL1; Islet-1; Islet1; ISLET1; P61371; Insulin gene enhancer protein ISL-1; ISL LIM homeobox 1; anti-ISL1 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
349
Applicable Applications for anti-ISL1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Notes
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Islet 1 (118-161aa DEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAE), identical to the related mouse and rat sequences.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Islet 1 was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- Islet 1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohiostchemistry)
(Islet 1 was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- Islet 1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
WB (Western Blot)
(Western blot analysis of Islet 1 expression in PC12 whole cell lysates (lane 1) and MCF-7 whole cell lysates (lane 2). Islet 1 at 39KD was detected using rabbit anti- Islet 1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)
WB (Western Blot)
(Western blot analysis of Islet 1 expression in PC12 whole cell lysates (lane 1) and MCF-7 whole cell lysates (lane 2). Islet 1 at 39KD was detected using rabbit anti- Islet 1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)
Related Product Information for anti-ISL1 antibody
Rabbit IgG polyclonal antibody for Insulin gene enhancer protein ISL-1(ISL1) detection.
Background: Insulin gene enhancer protein ISL-1 is a protein that in humans is encoded by the isl1 gene. This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.
Background: Insulin gene enhancer protein ISL-1 is a protein that in humans is encoded by the isl1 gene. This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.
References
1. "Entrez Gene: ISL1 ISL1 transcription factor, LIM/homeodomain, (islet-1)".
2. Gay F, Anglade I, Gong Z, Salbert G (October 2000). "The LIM/homeodomain protein islet-1 modulates estrogen receptor functions". Mol. Endocrinol. 14 (10): 1627-48.
3. Tanizawa Y, Riggs AC, Dagogo-Jack S, Vaxillaire M, Froguel P, Liu L, Donis-Keller H, Permutt MA (July 1994). "Isolation of the human LIM/homeodomain gene islet-1 and identification of a simple sequence repeat polymorphism [corrected]". Diabetes. 43 (7): 935-41.
2. Gay F, Anglade I, Gong Z, Salbert G (October 2000). "The LIM/homeodomain protein islet-1 modulates estrogen receptor functions". Mol. Endocrinol. 14 (10): 1627-48.
3. Tanizawa Y, Riggs AC, Dagogo-Jack S, Vaxillaire M, Froguel P, Liu L, Donis-Keller H, Permutt MA (July 1994). "Isolation of the human LIM/homeodomain gene islet-1 and identification of a simple sequence repeat polymorphism [corrected]". Diabetes. 43 (7): 935-41.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,036 Da
NCBI Official Full Name
insulin gene enhancer protein ISL-1
NCBI Official Synonym Full Names
ISL LIM homeobox 1
NCBI Official Symbol
ISL1
NCBI Official Synonym Symbols
Isl-1; ISLET1
NCBI Protein Information
insulin gene enhancer protein ISL-1
UniProt Protein Name
Insulin gene enhancer protein ISL-1
UniProt Gene Name
ISL1
UniProt Synonym Gene Names
Islet-1
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Product Notes
The ISL1 isl1 (Catalog #AAA46746) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Islet 1 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Islet 1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ISL1 isl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Islet 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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