IHC (Immunohiostchemistry)
(ISM1 Antibody (C-term) (#AAA284832) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ISM1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit ISM1 Polyclonal Antibody | anti-ISM1 antibody
ISM1 Antibody (C-term)
Gene Names
ISM1; ISM; Isthmin; C20orf82; bA149I18.1; dJ1077I2.1
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
ISM1, Antibody; ISM1 Antibody (C-term); Isthmin-1; ISM1; C20orf82; ISM; anti-ISM1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This ISM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 435-464 amino acids of human ISM1.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
435-464
Sequence Length
464
Applicable Applications for anti-ISM1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Acts as an angiogenesis inhibitor.
Cellular Location
Secreted.
Preparation and Storage
Maintain refrigerated at 2-8 degrees C for up to 2 weeks. For long term storage store at -20 degrees C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(ISM1 Antibody (C-term) (#AAA284832) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ISM1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(ISM1 Antibody (C-term) (#AAA284832) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ISM1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Anti-ISM1 Antibody (C-term) at 1:2000 dilution + Human placenta tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-ISM1 Antibody (C-term) at 1:2000 dilution + Human placenta tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Product Categories/Family for anti-ISM1 antibody
References
Simon-Sanchez, J., et al. Nat. Genet. 41(12):1308-1312(2009)
Pera, E.M., et al. Mech. Dev. 116 (1-2), 169-172 (2002)
Deloukas, P., et al. Nature 414(6866):865-871(2001)
Pera, E.M., et al. Mech. Dev. 116 (1-2), 169-172 (2002)
Deloukas, P., et al. Nature 414(6866):865-871(2001)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
isthmin-1
NCBI Official Synonym Full Names
isthmin 1, angiogenesis inhibitor
NCBI Official Symbol
ISM1
NCBI Official Synonym Symbols
ISM; Isthmin; C20orf82; bA149I18.1; dJ1077I2.1
NCBI Protein Information
isthmin-1
UniProt Protein Name
Isthmin-1
UniProt Gene Name
ISM1
UniProt Synonym Gene Names
C20orf82; ISM
UniProt Entry Name
ISM1_HUMAN
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Product Notes
The ISM1 ism1 (Catalog #AAA284832) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 435-464. The ISM1 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's ISM1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ISM1 ism1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ISM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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