IHC (Immunohistochemistry)
(Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.)
Goat ITGAM/CD11B Polyclonal Antibody | anti-ITGAM antibody
Goat anti-ITGAM/CD11B Antibody
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Reactivity
Tested: HumanExpected from sequence similarity: Human
Applications
ELISA, Immunofluorescence
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ITGAM/CD11B, Antibody; Goat anti-ITGAM/CD11B Antibody; ITGAM; integrin, alpha M (complement component 3 receptor 3 subunit); CD11B; CR3A; MAC-1; MAC1A; MGC117044; MO1A; Integrin, alpha-M (complement component receptor-3, alpha); antigen CD11B (p170); macrophage antigen, alpha polypeptide; antigen CD11b (p; ITGAM antibody; integrin; alpha M (complement component 3 receptor 3 subunit) antibody; CD11B antibody; CR3A antibody; MAC-1 antibody; MAC1A antibody; MGC117044 antibody; MO1A antibody; Integrin; alpha-M (complement component receptor-3; alpha) antibody; antigen CD11B (p170) antibody; macrophage antigen; alpha polypeptide antibody; antigen CD11b (p170) antibody; complement component receptor 3; alpha antibody; integrin alpha M antibody; alpha M (complement component receptor 3; CD11b (p170); macrophage antigen alpha polypeptide antibody; neutrophil adherence receptor alpha-M subunit antibody; ITGAM / CD11B; anti-ITGAM antibody
Host
Goat
Reactivity
Tested: Human
Expected from sequence similarity: Human
Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Applicable Applications for anti-ITGAM antibody
Peptide ELISA, Immunofluorescence (IF)
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000.
Immunohistochemistry (IHC): Paraffin embedded Human Tonsil. Recommended concentration: 6-8ug/mL.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U937 cells. Recommended concentration: 10 ug/ml.
Immunohistochemistry (IHC): Paraffin embedded Human Tonsil. Recommended concentration: 6-8ug/mL.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U937 cells. Recommended concentration: 10 ug/ml.
Immunogen
Peptide with sequence C-RNDGEDSYRTQ, from the internal region of the protein sequence according to NP_000623.2.
Note
The immunizing peptide represents part of the extra-cellular domain.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
IHC (Immunohistochemistry)
(Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.)
IHC (Immunohistochemistry)
(Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.)
IHC (Immunohistochemistry)
((8ug/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)
IHC (Immunohistochemistry)
((8ug/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
integrin alpha-M isoform 2
NCBI Official Synonym Full Names
integrin subunit alpha M
NCBI Official Symbol
ITGAM
NCBI Official Synonym Symbols
CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
NCBI Protein Information
integrin alpha-M
UniProt Protein Name
Integrin alpha-M
UniProt Gene Name
ITGAM
UniProt Synonym Gene Names
CD11B; CR3A
UniProt Entry Name
ITAM_HUMAN
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Product Notes
The ITGAM itgam (Catalog #AAA13654) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ITGAM/CD11B Antibody reacts with Tested: Human Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's ITGAM/CD11B can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA, Immunofluorescence (IF). Peptide ELISA: antibody detection limit dilution 1:16000. Immunohistochemistry (IHC): Paraffin embedded Human Tonsil. Recommended concentration: 6-8ug/mL. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U937 cells. Recommended concentration: 10 ug/ml. Researchers should empirically determine the suitability of the ITGAM itgam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ITGAM/CD11B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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