WB (Western Blot)
(CD11b Antibody (N-term) western blot analysis in A549,K562 cell line lysates (35ug/lane).This demonstrates the CD11b antibody detected the CD11b protein (arrow).)
Rabbit anti-Human CD11b Polyclonal Antibody | anti-ITGAM antibody
CD11b Antibody (N-term)
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CD11b, Antibody; CD11b Antibody (N-term); Integrin alpha-M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CD11b; ITGAM; CD11B; CR3A; anti-ITGAM antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CD11b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-282 amino acids from the N-terminal region of human CD11b.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.27 (varies by lot)
Sequence Positions
253-282
Sequence Length
1,153
Applicable Applications for anti-ITGAM antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(CD11b Antibody (N-term) western blot analysis in A549,K562 cell line lysates (35ug/lane).This demonstrates the CD11b antibody detected the CD11b protein (arrow).)
WB (Western Blot)
(CD11b Antibody (N-term) western blot analysis in A549,K562 cell line lysates (35ug/lane).This demonstrates the CD11b antibody detected the CD11b protein (arrow).)
FCM/FACS (Flow Cytometry)
(CD11b Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CD11b Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemistry)
(CD11b antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD11b antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(CD11b antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD11b antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-ITGAM antibody
CD11b is the integrin alpha M chain. Integrins
are heterodimeric integral membrane proteins composed of an alpha
chain and a beta chain. This I-domain containing alpha integrin
combines with the beta 2 chain (ITGB2) to form a leukocyte-specific
integrin referred to as macrophage receptor 1 ('Mac-1'), or
inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2
integrin is important in the adherence of neutrophils and monocytes
to stimulated endothelium, and also in the phagocytosis of
complement coated particles.
are heterodimeric integral membrane proteins composed of an alpha
chain and a beta chain. This I-domain containing alpha integrin
combines with the beta 2 chain (ITGB2) to form a leukocyte-specific
integrin referred to as macrophage receptor 1 ('Mac-1'), or
inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2
integrin is important in the adherence of neutrophils and monocytes
to stimulated endothelium, and also in the phagocytosis of
complement coated particles.
Product Categories/Family for anti-ITGAM antibody
References
Gjelstrup, L.C., et al. J. Immunol. 185(7):4154-4168(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Warchol, T., et al. DNA Cell Biol. (2010) In press :
Fan, Y., et al. J Eur Acad Dermatol Venereol (2010) In press :
Pliyev, B.K., et al. Biochem. Biophys. Res. Commun. 397(2):277-282(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Warchol, T., et al. DNA Cell Biol. (2010) In press :
Fan, Y., et al. J Eur Acad Dermatol Venereol (2010) In press :
Pliyev, B.K., et al. Biochem. Biophys. Res. Commun. 397(2):277-282(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
127179
NCBI Official Full Name
integrin alpha-M isoform 2
NCBI Official Synonym Full Names
integrin, alpha M (complement component 3 receptor 3 subunit)
NCBI Official Symbol
ITGAM
NCBI Official Synonym Symbols
CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
NCBI Protein Information
integrin alpha-M
UniProt Protein Name
Integrin alpha-M
UniProt Gene Name
ITGAM
UniProt Synonym Gene Names
CD11B; CR3A
UniProt Entry Name
ITAM_HUMAN
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Product Notes
The ITGAM itgam (Catalog #AAA289464) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 253-282. The CD11b Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD11b can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the ITGAM itgam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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