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IP (Immunoprecipitation)
(ITGB1 was immunoprecipitated using:; Lane A:0.5 mg hela Whole Cell Lysate; 2 uL anti-ITGB1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose.; Primary antibody:; Anti-ITGB1 rabbit polyclonal antibody,at 1:200 dilution; Secondary antibody:; Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution; Developed using the odssey technique.; Performed under reducing conditions.; Predicted band size: 88 kDa; Observed band size: 108 kDa)
Rabbit anti-Human ITGB1/Integrin beta 1 Polyclonal Antibody | anti-ITGB1 antibody
ITGB1/Integrin beta 1 Antibody, Rabbit PAb, Antigen Affinity Purified
Gene Names
ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Synonyms
ITGB1/Integrin beta 1, Antibody; ITGB1/Integrin beta 1 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-CD29 Antibody; Anti-FNRB Antibody; Anti-GPIIA Antibody; Anti-MDF2 Antibody; Anti-MSK12 Antibody; Anti-VLA-BETA Antibody; Anti-VLAB Antibody; anti-ITGB1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human Integrin beta 1
Applicable Applications for anti-ITGB1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Integrin beta 1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(ITGB1 was immunoprecipitated using:; Lane A:0.5 mg hela Whole Cell Lysate; 2 uL anti-ITGB1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose.; Primary antibody:; Anti-ITGB1 rabbit polyclonal antibody,at 1:200 dilution; Secondary antibody:; Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution; Developed using the odssey technique.; Performed under reducing conditions.; Predicted band size: 88 kDa; Observed band size: 108 kDa)
IP (Immunoprecipitation)
(ITGB1 was immunoprecipitated using:; Lane A:0.5 mg hela Whole Cell Lysate; 2 uL anti-ITGB1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose.; Primary antibody:; Anti-ITGB1 rabbit polyclonal antibody,at 1:200 dilution; Secondary antibody:; Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution; Developed using the odssey technique.; Performed under reducing conditions.; Predicted band size: 88 kDa; Observed band size: 108 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of ITGB1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human ITGB1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to cell membrane.)
IF (Immunofluorescence)
(Immunofluorescence staining of ITGB1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human ITGB1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to cell membrane.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human ITGB1 in human skin with rabbit polyclonal antibody (0.2 ug/mL, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human ITGB1 in human skin with rabbit polyclonal antibody (0.2 ug/mL, formalin-fixed paraffin embedded sections).)
WB (Western Blot)
(Anti-ITGB1 rabbit polyclonal antibody at 1:100 dilution.; Lane A: Hela Membrane Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.; Developed using the Odyssey technique.; Performed under reducing conditions.; Predicted band size:88 kDa; Observed band size:150 kDa)
WB (Western Blot)
(Anti-ITGB1 rabbit polyclonal antibody at 1:100 dilution.; Lane A: Hela Membrane Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.; Developed using the Odyssey technique.; Performed under reducing conditions.; Predicted band size:88 kDa; Observed band size:150 kDa)
Related Product Information for anti-ITGB1 antibody
ITGB1 (Integrin Subunit Beta 1) is a Protein Coding gene. This gene encodes a beta subunit, which is a type 1 transmembrane protein of the integrin beta chain family. ITGB1 is a heterodimeric cell-surface receptor involved in cell functions such as proliferation, migration, invasion, and survival. ITGB1 has been recognized to play a major role in tumor growth, invasion, and metastasis. Using luciferase assays, the researcher identified ITGB1 as a direct target of miR-134. ITGB1 is a direct target of miR-493-5p suggesting that ITGB1 and miR-493-5p may have potential prognostic value and may be useful as tumor biomarkers for the identification of NSCLC patients. Diseases associated with ITGB1 include Gallbladder Cancer and Breast Cancer.
Product Categories/Family for anti-ITGB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
88,415 Da
NCBI Official Full Name
integrin beta-1 isoform 1A
NCBI Official Synonym Full Names
integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
NCBI Official Symbol
ITGB1
NCBI Official Synonym Symbols
CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
NCBI Protein Information
integrin beta-1; glycoprotein IIa; integrin VLA-4 beta subunit; very late activation protein, beta polypeptide
UniProt Protein Name
Integrin beta-1
UniProt Gene Name
ITGB1
UniProt Synonym Gene Names
FNRB; MDF2; MSK12; GPIIA
UniProt Entry Name
ITB1_HUMAN
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Product Notes
The ITGB1 itgb1 (Catalog #AAA258598) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ITGB1/Integrin beta 1 Antibody, Rabbit PAb, Antigen Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ITGB1/Integrin beta 1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ITGB1 itgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ITGB1/Integrin beta 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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