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FCM/FACS (Flow Cytometry)
(ITPA Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit ITPA Polyclonal Antibody | anti-ITPA antibody
ITPA Antibody (N-term)
Gene Names
ITPA; My049; C20orf37; dJ794I6.3; HLC14-06-P
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat, Xenopus)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ITPA, Antibody; ITPA Antibody (N-term); Inosine triphosphate pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_03148}; ITPase {ECO:0000255|HAMAP-Rule:MF_03148}; Inosine triphosphatase {ECO:0000255|HAMAP-Rule:MF_03148}; 36119 {ECO:0000255|HAMAP-Rule:MF_03148}; Non-canonical purine NTP pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_03148}; Non-standard purine NTP pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_03148}; Nucleoside-triphosphate diphosphatase {ECO:0000255|HAMAP-Rule:MF_03148}; Nucleoside-triphosphate pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_03148}; NTPase {ECO:0000255|HAMAP-Rule:MF_03148}; Putative oncogene protein hlc14-06-p; ITPA {ECO:0000255|HAMAP-Rule:MF_03148}; C20orf37; anti-ITPA antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ITPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-51 amino acids from the N-terminal region of human ITPA.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
24-51
Sequence Length
153
Applicable Applications for anti-ITPA antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ITPA Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ITPA Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(ITPA Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ITPA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(ITPA Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ITPA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells, using ITPA Antibody (N-term). AAA287650 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells, using ITPA Antibody (N-term). AAA287650 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)
WB (Western Blot)
(Western blot analysis of lysates from A375, Hela, HepG2 cell line, mouse brain and rat liver tissue lysate(from left to right), using ITPA Antibody (N-term). AAA287650 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from A375, Hela, HepG2 cell line, mouse brain and rat liver tissue lysate(from left to right), using ITPA Antibody (N-term). AAA287650 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Related Product Information for anti-ITPA antibody
ITPA hydrolyzes inosine triphosphate and deoxyinosine triphosphate to the monophosphate nucleotide and diphosphate. The encoded protein, which is a member of the HAM1 NTPase protein family, is found in the cytoplasm and acts as a homodimer. Defects in the encoded protein can result in inosine triphosphate pyrophosphorylase deficiency. Two transcript variants encoding two different isoforms have been found for this gene.
Product Categories/Family for anti-ITPA antibody
References
Fellay, J., et al. Nature 464(7287):405-408(2010)
Herting, G., et al. Biochim. Biophys. Acta 1802(2):269-274(2010)
Kudo, M., et al. Drug Metab. Pharmacokinet. 24(6):557-564(2009)
Herting, G., et al. Biochim. Biophys. Acta 1802(2):269-274(2010)
Kudo, M., et al. Drug Metab. Pharmacokinet. 24(6):557-564(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21446
NCBI Official Full Name
inosine triphosphate pyrophosphatase isoform c
NCBI Official Synonym Full Names
inosine triphosphatase (nucleoside triphosphate pyrophosphatase)
NCBI Official Symbol
ITPA
NCBI Official Synonym Symbols
My049; C20orf37; dJ794I6.3; HLC14-06-P
NCBI Protein Information
inosine triphosphate pyrophosphatase
UniProt Protein Name
Inosine triphosphate pyrophosphatase
UniProt Gene Name
ITPA
UniProt Synonym Gene Names
ITPase; Inosine triphosphatase; NTPase
UniProt Entry Name
ITPA_HUMAN
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Product Notes
The ITPA itpa (Catalog #AAA287650) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 24-51. The ITPA Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's ITPA can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the ITPA itpa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ITPA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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