IHC (Immunohiostchemistry)
(Immunohistochemistry of IZUMO1 in human testis tissue with IZUMO1 antibody at 2.5 μg/mL.)
Rabbit anti-Human IZUMO1 Polyclonal Antibody | anti-IZUMO1 antibody
IZUMO1 Antibody
Gene Names
IZUMO1; OBF; IZUMO
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Affinity chromatography purified via peptide column.
Synonyms
IZUMO1, Antibody; IZUMO1 Antibody; Izumo sperm-egg fusion 1, IZUMO, Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, OBF, Sperm-specific protein izumo; anti-IZUMO1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
IZUMO1 antibody is human specific. At least four isoforms of IZUMO1 are known to exist; this antibody will detect the two longest isoforms.
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
350
Applicable Applications for anti-IZUMO1 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
IZUMO1 antibody was raised against an 18 amino acid peptide near the center of human IZUMO1
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
Human Testis Tissue Lysate
Conjugate
Unconjugated
MW Explanation
Transmembrane protein, also multiple other IZUMO1 antibodies also show a ~52kD band
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
IHC (Immunohiostchemistry)
(Immunohistochemistry of IZUMO1 in human testis tissue with IZUMO1 antibody at 2.5 μg/mL.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of IZUMO1 in human testis tissue with IZUMO1 antibody at 2.5 μg/mL.)
WB (Western Blot)
(Western blot analysis of IZUMO1 in human testis tissue lysate with IZUMO1 antibody at 1 μg/ml.)
WB (Western Blot)
(Western blot analysis of IZUMO1 in human testis tissue lysate with IZUMO1 antibody at 1 μg/ml.)
Related Product Information for anti-IZUMO1 antibody
IZUMO1, a sperm-specific protein, is essential for sperm-egg plasma membrane binding and fusion. It is involved in the fertilization process and in the fusion of the sperm with the egg (1,2). IZUMO1 is expressed as at least four isoforms produced by alternative splicing and contains one Ig-like (immunoglobulin-like) C2-type domain (3). Lack of IZUMO1 in males, due to homozygous mutation in the gene that encodes IZUMO1, results in sterility due to an inability to penetrate the plasma membrane of the egg (4).
Product Categories/Family for anti-IZUMO1 antibody
References
Inoue N, Hamada D, Kamikubo H, et al. Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion. Development 2013; 140:3221-9. Inoue N, Ikawa M, and Okabe M. The mechanism of sperm-egg interaction and the involvement of IZUMO1 in fusion. Asian J. Androl. 2011; 13:81-7. Xing WJ, Han BD, Wu Q, et al. Molecular cloning and characterization of Izumo1 gene from sheep and cashmere goat reveal alternative splicing. Mol. Biol. Rep. 2011; 38:1995-2006. Inoue N, Ikawa M, Isotani A, et al. The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs. Nature 2005; 434:234-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 35, 39, 42 kDa
Observed: 52 kDa
Observed: 52 kDa
NCBI Official Full Name
izumo sperm-egg fusion protein 1 isoform 1
NCBI Official Synonym Full Names
izumo sperm-egg fusion 1
NCBI Official Symbol
IZUMO1
NCBI Official Synonym Symbols
OBF; IZUMO
NCBI Protein Information
izumo sperm-egg fusion protein 1
UniProt Protein Name
Izumo sperm-egg fusion protein 1
UniProt Gene Name
IZUMO1
UniProt Synonym Gene Names
OBF
UniProt Entry Name
IZUM1_HUMAN
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Product Notes
The IZUMO1 izumo1 (Catalog #AAA40830) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IZUMO1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IZUMO1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the IZUMO1 izumo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IZUMO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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