WB (Western Blot)
(Western blot analysis of Phospho-JAK1 (Tyr1125) in lysates of MCF7 EGF, using Phospho-JAK1 (Tyr1125) Antibody.)
Rabbit JAK1 Polyclonal Antibody | anti-JAK1 antibody
Phospho-JAK1 (Tyr1125) Antibody
Gene Names
JAK1; JTK3; JAK1A; JAK1B
Reactivity
Human, Mouse, RatPredicted: Bovine, Horse, Sheep, Rabbit, Dog
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
JAK1, Antibody; Phospho-JAK1 (Tyr1125) Antibody; JAK 1; JAK 1A; JAK 1B; JAK-1; JAK1; JAK1_HUMAN; JAK1A; JAK1B; Janus kinase 1 (a protein tyrosine kinase); Janus kinase 1; JTK3; Tyrosine protein kinase JAK 1; Tyrosine protein kinase JAK1; Tyrosine-protein kinase JAK1; anti-JAK1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Bovine, Horse, Sheep, Rabbit, Dog
Predicted: Bovine, Horse, Sheep, Rabbit, Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-JAK1 (Tyr1125) Antibody detects endogenous levels of JAK1 only when phosphorylated at Tyr1125.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1154
Applicable Applications for anti-JAK1 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human JAK1 around the phosphorylation site of Tyr1125.
Tissue Specificity
Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
Subcellular Location
Endomembrane System. Wholly intracellular, possibly membrane associated.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of Phospho-JAK1 (Tyr1125) in lysates of MCF7 EGF, using Phospho-JAK1 (Tyr1125) Antibody.)
WB (Western Blot)
(Western blot analysis of Phospho-JAK1 (Tyr1125) in lysates of MCF7 EGF, using Phospho-JAK1 (Tyr1125) Antibody.)
IHC (Immunohiostchemistry)
(At 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohiostchemistry)
(At 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining mouse spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Related Product Information for anti-JAK1 antibody
Description: Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway (PubMed:7615558). Kinase partner for the interleukin (IL)-2 receptor (PubMed:11909529).
Post Translational Modifications: Autophosphorylated (PubMed:7615558). Phosphorylated on tyrosine residues in response to interferon gamma signaling (PubMed:7615558). Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling (PubMed:11909529). Ubiquitinated by RNF125; leading to its degradation by the proteasome.
Subunit Structure: Interacts with IL31RA (PubMed:15194700). Interacts with IFNAR2 (PubMed:7759950). Interacts with IFNGR1 (PubMed:7615558). Interacts with JAKMIP1 (PubMed:15277531). Interacts with SHB (PubMed:12200137). Interacts with FER (By similarity).
Similarity: Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1. The FERM domain mediates interaction with JAKMIP1. Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Post Translational Modifications: Autophosphorylated (PubMed:7615558). Phosphorylated on tyrosine residues in response to interferon gamma signaling (PubMed:7615558). Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling (PubMed:11909529). Ubiquitinated by RNF125; leading to its degradation by the proteasome.
Subunit Structure: Interacts with IL31RA (PubMed:15194700). Interacts with IFNAR2 (PubMed:7759950). Interacts with IFNGR1 (PubMed:7615558). Interacts with JAKMIP1 (PubMed:15277531). Interacts with SHB (PubMed:12200137). Interacts with FER (By similarity).
Similarity: Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1. The FERM domain mediates interaction with JAKMIP1. Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 130 kDa
Predicted: 134 kDa
Predicted: 134 kDa
NCBI Official Full Name
tyrosine-protein kinase JAK1 isoform 1
NCBI Official Synonym Full Names
Janus kinase 1
NCBI Official Symbol
JAK1
NCBI Official Synonym Symbols
JTK3; JAK1A; JAK1B
NCBI Protein Information
tyrosine-protein kinase JAK1
UniProt Protein Name
Tyrosine-protein kinase JAK1
UniProt Gene Name
JAK1
UniProt Synonym Gene Names
JAK1A; JAK1B; JAK-1
UniProt Entry Name
JAK1_HUMAN
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Product Notes
The JAK1 jak1 (Catalog #AAA328419) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-JAK1 (Tyr1125) Antibody reacts with Human, Mouse, Rat Predicted: Bovine, Horse, Sheep, Rabbit, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's JAK1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the JAK1 jak1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JAK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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