Rabbit JAK2 Polyclonal Antibody | anti-JAK2 antibody

JAK2(Phospho-Tyr221) Antibody

Cat. Num. AAA304732

• Gene Names: JAK2; JTK10; THCYT3
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Synonyms: JAK2, Antibody; JAK2(Phospho-Tyr221) Antibody; JTK10; THCYT3; anti-JAK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of JAK2 only when phosphorylated at tyrosine 221.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1132

• Applicable Applications for anti-JAK2 antibody: IHC (Immunohistochemistry)
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of tyrosine 221(Q-D-Y(p)-H-I)derived from Human JAK2.
• Modification: Phospho
• Target Name: JAK2
• Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.; Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide.; Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide
• Preparation and Storage: Store at -20 degree C

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using JAK2 (phospho-Tyr221) antibody ().)

Related Product Information for anti-JAK2 antibody

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.

Product Categories/Family for anti-JAK2 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4826776
• NCBI GeneID: 3717
• NCBI Accession #: NP_004963.1
• NCBI GenBank Nucleotide #: NM_004972.3
• UniProt Accession #: O60674; O14636; O75297
• Molecular Weight: Predicted MW: 125kd
• NCBI Official Full Name: tyrosine-protein kinase JAK2
• NCBI Official Synonym Full Names: Janus kinase 2
• NCBI Official Symbol: JAK2
• NCBI Official Synonym Symbols: JTK10; THCYT3
• NCBI Protein Information: tyrosine-protein kinase JAK2
• UniProt Protein Name: Tyrosine-protein kinase JAK2
• UniProt Gene Name: JAK2
• UniProt Synonym Gene Names: JAK-2
• UniProt Entry Name: JAK2_HUMAN