WB (Western Blot)
(Detection of human JAK2 by western blot. Samples: Whole cell lysate (50 ug) from HEL 92.1.7, MDA-MB-435, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JAK2 antibody (AAA211792 lot 2) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human JAK2 Polyclonal Antibody | anti-JAK2 antibody
Rabbit anti-JAK2 Antibody, Affinity Purified
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
JAK2, Antibody; Rabbit anti-JAK2 Antibody, Affinity Purified; tyrosine-protein kinase JAK2; JAK-2; Janus kinase 2; Janus kinase 2 (a protein tyrosine kinase); JTK10; THCYT3; Tyrosine-protein kinase JAK2; anti-JAK2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-JAK2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 1082 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human JAK2 by western blot. Samples: Whole cell lysate (50 ug) from HEL 92.1.7, MDA-MB-435, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JAK2 antibody (AAA211792 lot 2) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human JAK2 by western blot. Samples: Whole cell lysate (50 ug) from HEL 92.1.7, MDA-MB-435, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JAK2 antibody (AAA211792 lot 2) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human JAK2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEL 92.1.7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JAK2 antibody (AAA211792 lot 2) used for IP at 6 ug per reaction. JAK2 was also immunoprecipitated by a previous lot of this antibody (AAA211792 lot 1) and a second antibody against a different epitope of JAK2 (Antibody 2). For blotting immunoprecipitated JAK2, AAA211792 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human JAK2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEL 92.1.7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JAK2 antibody (AAA211792 lot 2) used for IP at 6 ug per reaction. JAK2 was also immunoprecipitated by a previous lot of this antibody (AAA211792 lot 1) and a second antibody against a different epitope of JAK2 (Antibody 2). For blotting immunoprecipitated JAK2, AAA211792 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human JAK2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-JAK2 (Cat. No. AAA211792) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human JAK2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-JAK2 (Cat. No. AAA211792) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-JAK2 antibody
JAK2 (Janus kinase 2) was originally cloned via screening of mouse cDNA libraries with PCR generated probes representing highly conserved elements in the protein tyrosine kinase (PTK) family. JAK2, as well as JAK, was named after the Roman god, Janus, the god of gates or doors, and who is depicted as having two faces which face opposite directions. JAK kinases bear two nearly identical phosphate transferring domains and function to transduce signals from type I and type II cytokine receptors for the activation of STAT (signal transducers and activators of transcription) transcription factors.
Product Categories/Family for anti-JAK2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
130,674 Da
NCBI Official Full Name
tyrosine-protein kinase JAK2
NCBI Official Synonym Full Names
Janus kinase 2
NCBI Official Symbol
JAK2
NCBI Official Synonym Symbols
JTK10; THCYT3
NCBI Protein Information
tyrosine-protein kinase JAK2; JAK-2; Janus kinase 2 (a protein tyrosine kinase)
UniProt Protein Name
Tyrosine-protein kinase JAK2
UniProt Gene Name
JAK2
UniProt Synonym Gene Names
JAK-2
UniProt Entry Name
JAK2_HUMAN
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Product Notes
The JAK2 jak2 (Catalog #AAA211792) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-JAK2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JAK2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the JAK2 jak2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JAK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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