WB (Western Blot)
(Anti-JAM-A rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:33 kDaObserved band size:38 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human JAM-A/Junctional Adhesion Molecule A Polyclonal Antibody | anti-F11R antibody
Anti-JAM-A/Junctional Adhesion Molecule A Antibody, Rabbit Polyclonal
Gene Names
F11R; JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
JAM-A/Junctional Adhesion Molecule A, Antibody; Anti-JAM-A/Junctional Adhesion Molecule A Antibody, Rabbit Polyclonal; JAM-A/F11R Antibody, Rabbit PAb, Antigen Affinity Purified; F11 receptor; Anti-CD321 Antibody; Anti-JAM Antibody; Anti-JAM1 Antibody; Anti-JAMA Antibody; Anti-JCAM Antibody; Anti-KAT Antibody; Anti-PAM-1 Antibody; anti-F11R antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human JAM-A/PAM-1/CD321
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-F11R antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Human JAM-A protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human JAM-A extracellular domain (rh JAM-A). JAM-A specific IgG was purified by human JAM-A affinity chromatography
.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-JAM-A rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:33 kDaObserved band size:38 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-JAM-A rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:33 kDaObserved band size:38 kDa(We are unsure as to the identity of these extra bands.))
IP (Immunoprecipitation)
(JAM-A was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate0.5 uL anti-JAM-A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-JAM-A rabbit polyclonal antibody,at 1:1000 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 39 kDaObserved band size: 39 kDa)
IP (Immunoprecipitation)
(JAM-A was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate0.5 uL anti-JAM-A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-JAM-A rabbit polyclonal antibody,at 1:1000 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 39 kDaObserved band size: 39 kDa)
Related Product Information for anti-F11R antibody
Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.
References
Guglielmi KM, et al. (2007) Reovirus binding determinants in junctional adhesion molecule-A. J Biol Chem. 282(24): 17930-40.Yeung D, et al. (2008) Decreased junctional adhesion molecule-A expression during blood-brain barrier breakdown. Acta Neuropathol. 115(6): 635-42.Ong KL, et al. (2009) Elevated plasma level of soluble F11 receptor/junctional adhesion molecule-A (F11R/JAM-A) in hypertension. Am J Hypertens. 22(5): 500-5.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,583 Da
NCBI Official Full Name
junctional adhesion molecule A
NCBI Official Synonym Full Names
F11 receptor
NCBI Official Symbol
F11R
NCBI Official Synonym Symbols
JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1
NCBI Protein Information
junctional adhesion molecule A; platelet F11 receptor; platelet adhesion molecule 1; junctional adhesion molecule 1
UniProt Protein Name
Junctional adhesion molecule A
UniProt Gene Name
F11R
UniProt Synonym Gene Names
JAM1; JCAM; JAM-A; JAM-1; PAM-1
UniProt Entry Name
JAM1_HUMAN
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Product Notes
The F11R f11r (Catalog #AAA254667) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-JAM-A/Junctional Adhesion Molecule An Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JAM-A/Junctional Adhesion Molecule A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the F11R f11r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JAM-A/Junctional Adhesion Molecule A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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