WB (Western Blot)
(Detection of human JARID1B by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1B antibody AAA211640 (lot AAA211640-3) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human JARID1B Polyclonal Antibody | anti-JARID1B antibody
Rabbit anti-JARID1B Antibody, Affinity Purified
Gene Names
KDM5B; CT31; PLU1; PUT1; PLU-1; JARID1B; PPP1R98; RBBP2H1A
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
JARID1B, Antibody; Rabbit anti-JARID1B Antibody, Affinity Purified; PLU-1; retinoblastoma-binding protein 2 homolog 1; CT31; RBP2-H1; retinoblastoma-binding protein 2, homolog 1A; RBBP2H1A; putative DNA/chromatin binding motif; PUT1; protein phosphatase 1, regulatory subunit 98; PPP1R98; MRT65; lysine-specific demethylase 5B; lysine (K)-specific demethylase 5B; jumonji/ARID domain-containing protein 1B; jumonji, AT rich interactive domain 1B; histone demethylase JARID1B; cancer/testis antigen 31; JARID1B; PLU1; Lysine-specific demethylase 5B; lysine demethylase 5B; anti-JARID1B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-JARID1B antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1494 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human JARID1B by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1B antibody AAA211640 (lot AAA211640-3) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human JARID1B by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1B antibody AAA211640 (lot AAA211640-3) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human JARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JARID1B antibody AAA211640 (lot AAA211640-3) used for IP at 6 ug per reaction. JARID1B was also immunoprecipitated by a previous lot of this antibody (lot AAA211640-2). For blotting immunoprecipitated Jarid1B, AAA211640 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human JARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JARID1B antibody AAA211640 (lot AAA211640-3) used for IP at 6 ug per reaction. JARID1B was also immunoprecipitated by a previous lot of this antibody (lot AAA211640-2). For blotting immunoprecipitated Jarid1B, AAA211640 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IHC (Immunohistochemistry)
(Detection of human JARID1B by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Rabbit anti-JARID1B antibody (AAA211640 lot 3) used at 1:500 (2ug/ml). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human JARID1B by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Rabbit anti-JARID1B antibody (AAA211640 lot 3) used at 1:500 (2ug/ml). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for anti-JARID1B antibody
JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer.
Product Categories/Family for anti-JARID1B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
179,409 Da
NCBI Official Full Name
Homo sapiens lysine (K)-specific demethylase 5B (KDM5B), mRNA
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 5B
NCBI Official Symbol
KDM5B
NCBI Official Synonym Symbols
CT31; PLU1; PUT1; PLU-1; JARID1B; PPP1R98; RBBP2H1A
NCBI Protein Information
lysine-specific demethylase 5B; RBP2-H1; cancer/testis antigen 31; histone demethylase JARID1B; jumonji, AT rich interactive domain 1B; jumonji/ARID domain-containing protein 1B; protein phosphatase 1, regulatory subunit 98; putative DNA/chromatin binding
UniProt Protein Name
Lysine-specific demethylase 5B
UniProt Gene Name
KDM5B
UniProt Synonym Gene Names
JARID1B; PLU1; RBBP2H1; CT31; RBP2-H1
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Product Notes
The JARID1B kdm5b (Catalog #AAA211640) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-JARID1B Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JARID1B can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the JARID1B kdm5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JARID1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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