WB (Western Blot)
(Detection of human SMCY/JARID1D by western blot. Samples: Whole cell lysate (15 ug) from Jurkat, HDLM-2, A-549, 786-O, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMCY/JARID1D antibody AAA211617 (lot AAA211617-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human JARID1D/SMCY Polyclonal Antibody | anti-JARID1D/SMCY antibody
Rabbit anti-JARID1D/SMCY Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
JARID1D/SMCY, Antibody; Rabbit anti-JARID1D/SMCY Antibody, Affinity Purified; HY; lysine (K)-specific demethylase 5D; Smcy homolog, Y-linked; SMCY; SMC homolog, Y chromosome; selected mouse cDNA on Y, human homolog of; protein SmcY; lysine-specific demethylase 5D; jumonji/ARID domain-containing protein 1D; Jumonji, AT rich interactive domain 1D (RBP2-like); JARID1D; H-Y; histone demethylase JARID1D; histocompatibility Y antigen; epididymis secretory sperm binding protein; HYA; Lysine-specific demethylase 5D; lysine demethylase 5D; anti-JARID1D/SMCY antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-JARID1D/SMCY antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1489 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SMCY/JARID1D by western blot. Samples: Whole cell lysate (15 ug) from Jurkat, HDLM-2, A-549, 786-O, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMCY/JARID1D antibody AAA211617 (lot AAA211617-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human SMCY/JARID1D by western blot. Samples: Whole cell lysate (15 ug) from Jurkat, HDLM-2, A-549, 786-O, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMCY/JARID1D antibody AAA211617 (lot AAA211617-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human SMCY/JARID1D by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMCY/JARID1D antibody AAA211617 (lot AAA211617-4) used for IP at 3 ug per reaction. SMCY/JARID1D was also immunoprecipitated by rabbit anti-SMCY/JARID1D antibody BL6690. For blotting immunoprecipitated SMCY/JARID1D, AAA211617 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human SMCY/JARID1D by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMCY/JARID1D antibody AAA211617 (lot AAA211617-4) used for IP at 3 ug per reaction. SMCY/JARID1D was also immunoprecipitated by rabbit anti-SMCY/JARID1D antibody BL6690. For blotting immunoprecipitated SMCY/JARID1D, AAA211617 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-JARID1D/SMCY antibody
JARID1D is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. JARID1D maps to the azoospermia factor region (AZF) on the Y chromosome and thus is also known as SMCY (selected mouse cDNA on Y chromosome) homolog. Jarid1D displays histone H3K4 demethylase activity and may have male specific functions.
Product Categories/Family for anti-JARID1D/SMCY antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The JARID1D/SMCY kdm5d (Catalog #AAA211617) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-JARID1D/SMCY Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JARID1D/SMCY can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the JARID1D/SMCY kdm5d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JARID1D/SMCY, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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