Rabbit anti-Human JIP1 Polyclonal Antibody | anti-MAPK8IP1 antibody

JIP1 Antibody (C-term)

Cat. Num. AAA289049

• Gene Names: MAPK8IP1; IB1; JIP1; JIP-1; PRKM8IP
• Reactivity: Human
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: JIP1, Antibody; JIP1 Antibody (C-term); C-Jun-amino-terminal kinase-interacting protein 1; JIP-1; JNK-interacting protein 1; Islet-brain 1; IB-1; JNK MAP kinase scaffold protein 1; Mitogen-activated protein kinase 8-interacting protein 1; MAPK8IP1; IB1; JIP1; PRKM8IP; anti-MAPK8IP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This JIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 470-498 amino acids from the C-terminal region of human JIP1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 470-498
• Sequence Length: 711

• Applicable Applications for anti-MAPK8IP1 antibody: IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of JIP1 Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

FCM/FACS (Flow Cytometry)

(JIP1 Antibody (C-term) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with JIP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of JIP1 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). JIP1 (arrow) was detected using the purified Pab.)

Related Product Information for anti-MAPK8IP1 antibody

JIP1 is a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2.

References

Mooser,V., et.al., Genomics 55 (2), 202-208 (1999)

NCBI and Uniprot Product Information

• NCBI GI #: 4885433
• NCBI GeneID: 9479
• NCBI Accession #: NP_005447.1
• NCBI GenBank Nucleotide #: NM_005456.3
• UniProt Accession #: Q9UQF2; O43407; D3DQP4
• Molecular Weight: 77524
• NCBI Official Full Name: C-Jun-amino-terminal kinase-interacting protein 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8 interacting protein 1
• NCBI Official Symbol: MAPK8IP1
• NCBI Official Synonym Symbols: IB1; JIP1; JIP-1; PRKM8IP
• NCBI Protein Information: C-Jun-amino-terminal kinase-interacting protein 1
• UniProt Protein Name: C-Jun-amino-terminal kinase-interacting protein 1
• UniProt Gene Name: MAPK8IP1
• UniProt Synonym Gene Names: IB1; JIP1; PRKM8IP; JIP-1; JNK-interacting protein 1; IB-1
• UniProt Entry Name: JIP1_HUMAN