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WB (Western Blot)
(Detection of mouse JLP by western blot. Samples: Whole cell lysate from TCKM (50ug), Renca (50ug), and CT26 (50ug) cells. Antibodies: Affinity purified rabbit anti-JLP antibody AAA210891 (lot AAA210891-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse JLP Polyclonal Antibody | anti-JLP antibody
Rabbit anti-JLP Antibody, Affinity Purified
Gene Names
SPAG9; JLP; CT89; HLC4; HLC6; JIP4; PHET; PIG6; HLC-6; JIP-4
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
JLP, Antibody; Rabbit anti-JLP Antibody, Affinity Purified; Sperm-associated antigen 9; Max-binding protein; mitogen-activated protein kinase 8-interacting protein 4; PHET; PIG6; proliferation-inducing gene 6; Proliferation-inducing protein 6; protein highly expressed in testis; sperm surface protein; lung cancer oncogene 4; Sperm-specific protein; sunday driver 1; c-Jun NH2-terminal kinase-associated leucine zipper protein; sperm associated antigen 9 variant 5; HLC-6; cancer/testis antigen 89; C-Jun-amino-terminal kinase-interacting protein 4; CT89; HLC6; JNK-interacting protein 4; human lung cancer oncogene 6 protein; JIP4; JIP-4; JLP; JNK interacting protein; JNK/SAPK-associated protein; JNK-associated leucine-zipper protein; HLC4; sperm associated antigen 9; anti-JLP antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-JLP antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 775 and 825
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse JLP by western blot. Samples: Whole cell lysate from TCKM (50ug), Renca (50ug), and CT26 (50ug) cells. Antibodies: Affinity purified rabbit anti-JLP antibody AAA210891 (lot AAA210891-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of mouse JLP by western blot. Samples: Whole cell lysate from TCKM (50ug), Renca (50ug), and CT26 (50ug) cells. Antibodies: Affinity purified rabbit anti-JLP antibody AAA210891 (lot AAA210891-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human JLP by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug) and HEK293T (50ug) cells. Antibodies: Affinity purified rabbit anti-JLP antibody AAA210891 (lot AAA210891-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human JLP by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug) and HEK293T (50ug) cells. Antibodies: Affinity purified rabbit anti-JLP antibody AAA210891 (lot AAA210891-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human JLP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JLP antibody AAA210891 (lot AAA210891-2) used for IP at 6 ug/mg lysate. JLP was also immunoprecipitated by a previous lot (AAA210891-1) of this antibody. For blotting immunoprecipitated JLP, AAA210891 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human JLP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JLP antibody AAA210891 (lot AAA210891-2) used for IP at 6 ug/mg lysate. JLP was also immunoprecipitated by a previous lot (AAA210891-1) of this antibody. For blotting immunoprecipitated JLP, AAA210891 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human JLP by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-JLP (Cat. No. AAA210891 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human JLP by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-JLP (Cat. No. AAA210891 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-JLP antibody
JLP (JNK-interacting leucine zipper protein) has been identified as a scaffolding protein that functions to position mitogen-activated protein kinases (MAPKs) into specific signaling modules so that successive phosphorylation events of the MAPK pathway can occur. JLP is also known as sperm associated antigen 9 (SPAG9) due to its abundant expression in testicular haploid germ cells.
Product Categories/Family for anti-JLP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
128,607 Da
NCBI Official Full Name
C-Jun-amino-terminal kinase-interacting protein 4 isoform 3
NCBI Official Synonym Full Names
sperm associated antigen 9
NCBI Official Symbol
SPAG9
NCBI Official Synonym Symbols
JLP; CT89; HLC4; HLC6; JIP4; PHET; PIG6; HLC-6; JIP-4
NCBI Protein Information
C-Jun-amino-terminal kinase-interacting protein 4; sunday driver 1; Max-binding protein; sperm surface protein; lung cancer oncogene 4; JNK interacting protein; cancer/testis antigen 89; JNK/SAPK-associated protein; proliferation-inducing gene 6; protein
UniProt Protein Name
C-Jun-amino-terminal kinase-interacting protein 4
UniProt Gene Name
SPAG9
UniProt Synonym Gene Names
HSS; KIAA0516; MAPK8IP4; SYD1; JIP-4; JNK-interacting protein 4; CT89; HLC-6; JLP; PHET
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Product Notes
The JLP spag9 (Catalog #AAA210891) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-JLP Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's JLP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the JLP spag9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JLP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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