IP (Immunoprecipitation)
(Detection of human JMY by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JMY antibody AAA211931 used for IP at 3 ug/mg lysate. JMY was less efficiently immunoprecipitated by rabbit anti-JMY antibody which recognizes a downstream epitope. For blotting immunoprecipitated JMY, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human JMY Polyclonal Antibody | anti-JMY antibody
Rabbit anti-JMY Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
JMY, Antibody; Rabbit anti-JMY Antibody, Affinity Purified; WHDC1L3; WHAMM2; junction-mediating and-regulatory protein; WAS protein homology region 2 domain containing 1-like 3; Junction-mediating and-regulatory protein; junction mediating and regulatory protein, p53 cofactor; anti-JMY antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-JMY antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Mouse
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human JMY by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JMY antibody AAA211931 used for IP at 3 ug/mg lysate. JMY was less efficiently immunoprecipitated by rabbit anti-JMY antibody which recognizes a downstream epitope. For blotting immunoprecipitated JMY, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human JMY by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JMY antibody AAA211931 used for IP at 3 ug/mg lysate. JMY was less efficiently immunoprecipitated by rabbit anti-JMY antibody which recognizes a downstream epitope. For blotting immunoprecipitated JMY, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human JMY by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-JMY (Cat. No. AAA211931 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human JMY by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-JMY (Cat. No. AAA211931 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-JMY antibody
Junction-mediating and-regulatory protein (JMY) acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In the nucleus, JMY acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300 and increases p53/TP53-dependent transcription and apoptosis suggesting an important role in p53/TP53 stress response such as DNA damage. In the cytoplasm, JMY acts as a nucleation-promoting factor for both branched and un-branched actin filaments. It activates the Arp2/3 complex to induce branched actin filament networks and also catalyzes actin polymerization in the absence of Arp2/3, creating un-branched filaments [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8N9B5].
Product Categories/Family for anti-JMY antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The JMY jmy (Catalog #AAA211931) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-JMY Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JMY can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the JMY jmy for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JMY, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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