IF (Immunofluorescence)
(Fluorescent confocal image of U251 cell stained with JUN Antibody (Center T93). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with JUN primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). JUN immunoreactivity is localized to Nucleus significantly.)
Rabbit JUN Polyclonal Antibody | anti-JUN antibody
JUN Antibody (Center T93)
Gene Names
JUN; AP1; AP-1; c-Jun
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Pig, Rat)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
JUN, Antibody; JUN Antibody (Center T93); Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; JUN; anti-JUN antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Pig, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This JUN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-98 amino acids from the Central region of human JUN.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
72-98
Sequence Length
331
Applicable Applications for anti-JUN antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of U251 cell stained with JUN Antibody (Center T93). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with JUN primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). JUN immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of U251 cell stained with JUN Antibody (Center T93). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with JUN primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). JUN immunoreactivity is localized to Nucleus significantly.)
WB (Western Blot)
(JUN Antibody (Center T93) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the JUN antibody detected the JUN protein (arrow).)
WB (Western Blot)
(JUN Antibody (Center T93) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the JUN antibody detected the JUN protein (arrow).)
Related Product Information for anti-JUN antibody
This gene is the putative transforming gene of avian
sarcoma virus 17. It encodes a protein which is highly similar to
the viral protein, and which interacts directly with specific
target DNA sequences to regulate gene expression. This gene is
intronless and is mapped to 1p32-p31, a chromosomal region involved
in both translocations and deletions in human malignancies.
sarcoma virus 17. It encodes a protein which is highly similar to
the viral protein, and which interacts directly with specific
target DNA sequences to regulate gene expression. This gene is
intronless and is mapped to 1p32-p31, a chromosomal region involved
in both translocations and deletions in human malignancies.
Product Categories/Family for anti-JUN antibody
References
Gonsalves, C., et al. J. Immunol. 185(10):6253-6264(2010)
Bozec, A., et al. J. Cell Biol. 190(6):1093-1106(2010)
Machida, K., et al. Hepatology 52(2):480-492(2010)
Madi, A., et al. BMC Microbiol. 10, 215 (2010) :
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
Bozec, A., et al. J. Cell Biol. 190(6):1093-1106(2010)
Machida, K., et al. Hepatology 52(2):480-492(2010)
Madi, A., et al. BMC Microbiol. 10, 215 (2010) :
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35676
NCBI Official Full Name
transcription factor AP-1
NCBI Official Synonym Full Names
jun proto-oncogene
NCBI Official Symbol
JUN
NCBI Official Synonym Symbols
AP1; AP-1; c-Jun
NCBI Protein Information
transcription factor AP-1
UniProt Protein Name
Transcription factor AP-1
UniProt Gene Name
JUN
UniProt Synonym Gene Names
AP1
UniProt Entry Name
JUN_HUMAN
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Product Notes
The JUN jun (Catalog #AAA286224) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 72-98. The JUN Antibody (Center T93) reacts with Human (Predicted Reactivity: Bovine, Mouse, Pig, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's JUN can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the JUN jun for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JUN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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