WB (Western Blot)
(Western blot analysis of K0776 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). K0776 (arrow) was detected using the purified Pab.)
Rabbit K0776 Polyclonal Antibody | anti-UFL1 antibody
K0776 Antibody (Center)
Gene Names
UFL1; NLBP; RCAD; Maxer; KIAA0776
Reactivity
HumanPredicted Reactivity: Rat
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
K0776, Antibody; K0776 Antibody (Center); E3 UFM1-protein ligase 1; 632-; LZAP-binding protein; UFL1; KIAA0776; NLBP; anti-UFL1 antibody
Host
Rabbit
Reactivity
Human
Predicted Reactivity: Rat
Predicted Reactivity: Rat
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This K0776 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 426-455 amino acids from the Central region of human K0776.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
426-455
Sequence Length
794
Applicable Applications for anti-UFL1 antibody
ELISA, WB (Western Blot)
Function
E3 protein ligase that mediates ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to lysine residues on target proteins, and which plays a key role in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress (PubMed: 20018847, PubMed: , PubMed: ,PubMed: ,PubMed: ). In response to endoplasmic reticulum stress, recruited to the endoplasmic reticulum membrane by DDRGK1, and mediates ufmylation of proteins such as RPN1 and RPL26/uL24, thereby promoting reticulophagy of endoplasmic reticulum sheets (PubMed: ).Ufmylation-dependent reticulophagy inhibits the unfolded protein response (UPR)via ERN1/IRE1-alpha (PubMed: ,PubMed: href="http://www.uniprot.org/citations/32160526" target="_blank">32160526). Ufmylation in response to endoplasmic reticulum stress is essential for processes such as hematopoiesis, blood vessel morphogenesis or inflammatory response (PubMed: ). Regulates inflammation in response to endoplasmic reticulum stress by promoting reticulophagy, leading to inhibit the activityof the NF-kappa-B transcription factor (By similarity). Mediates ufmylation of DDRGK1 and CDK5RAP3; the role of these modifications is however unclear: as both DDRGK1 and CDK5RAP3 act as substrate adapters for ufmylation, it is uncertain whether ufmylation of these proteins is a collateral effect or is required for ufmylation (PubMed: , PubMed: ). Catalyzes ufmylation of various subunits of the ribosomal complex or associated components, such as RPS3/uS3, RPS20/uS10, RPL10/uL16, RPL26/uL24 and EIF6 (By similarity). Anchors CDK5RAP3 in the cytoplasm, preventing its translocation to the nucleus which allows expression of the CCND1 cyclin and progression of cells through the G1/S transition (PubMed: 20531390). Also involved in the response to DNA damage: recruited to double-strand break sites following DNA damage and mediates monoufmylation of histone H4 (PubMed: ). Catalyzes ufmylation of TRIP4, thereby playing a role in nuclear receptor- mediated transcription (PubMed: ). Required for hematopoietic stem cell function and hematopoiesis (By similarity). Required for cardiac homeostasis (By similarity).
Cellular Location
Endoplasmic reticulum membrane.Cytoplasm, cytosol Nucleus. Chromosome. Note=Recruited to double-strand breaks by the MRE11-RAD50-NBN (MRN) complex following DNA damage.
Tissue Location
Ubiquitously expressed, with a high expression in liver (at protein level)(PubMed:20018847).Low expression in several invasive hepato cellular carcinomas, such Hep-G2, Hep 3B2.1-7, HLE and PLC (PubMed: 20018847).
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of K0776 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). K0776 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of K0776 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). K0776 (arrow) was detected using the purified Pab.)
Product Categories/Family for anti-UFL1 antibody
References
Kwon, J., et al. J. Biol. Chem. 285(16):12232-12240(2010)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Mungall, A.J., et al. Nature 425(6960):805-811(2003)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Mungall, A.J., et al. Nature 425(6960):805-811(2003)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
89595
NCBI Official Full Name
E3 UFM1-protein ligase 1
NCBI Official Synonym Full Names
UFM1-specific ligase 1
NCBI Official Symbol
UFL1
NCBI Official Synonym Symbols
NLBP; RCAD; Maxer; KIAA0776
NCBI Protein Information
E3 UFM1-protein ligase 1
UniProt Protein Name
E3 UFM1-protein ligase 1
UniProt Gene Name
UFL1
UniProt Entry Name
UFL1_HUMAN
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Product Notes
The UFL1 ufl1 (Catalog #AAA283652) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 426-455. The K0776 Antibody (Center) reacts with Human Predicted Reactivity: Rat and may cross-react with other species as described in the data sheet. AAA Biotech's K0776 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the UFL1 ufl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "K0776, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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