IF (Immunofluorescence)
(AAA323484 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA323484) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
Rabbit anti-Human, Mouse K2C1 Polyclonal Antibody | anti-K2C1 antibody
K2C1 Antibody
Gene Names
KRT1; K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Synonyms
K2C1, Antibody; K2C1 Antibody; 67 kDa cytokeratin; CK-1; CK1; Cytokeratin 1; Cytokeratin 19; Cytokeratin 8; Cytokeratin-1; EHK; EHK1; epidermolytic hyperkeratosis 1; EPPK; Hair alpha protein; K1; K2C1_HUMAN; KB1; Keratin 1; keratin 1, type II; Keratin 19; Keratin 8; Keratin; keratin, type II cytoskeletal 1; Keratin-1; Krt1; KRT19; KRT1A; KRT8; KRTA; NEPPK; type II cytoskeletal 1; Type-II keratin Kb1; anti-K2C1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
K2C1 Antibody detects endogenous levels of total K2C1.
Purity/Purification
Peptide affinity purification
Form/Format
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-K2C1 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human K2C1, corresponding to a region within N-terminal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA323484 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA323484) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
IF (Immunofluorescence)
(AAA323484 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA323484) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA323484 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA323484 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of K2C1 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of K2C1 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-K2C1 antibody
Function: May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
Subunit Structure: Heterotetramer of two type I and two type II keratins. Heterodimer with KRT10 (PubMed:27595935). Two heterodimers of KRT1 and KRT10 form a heterotetramer (PubMed:27595935). Interacts with ITGB1 in the presence of RACK1 and SRC, and with RACK1 (PubMed:17956333). Interacts with C1QBP; the association represents a cell surface kininogen receptor (PubMed:21544310). Interacts with EPPK1; interaction is dependent of higher-order structure of intermediate filament (PubMed:16923132).
Post-translational Modifications: Undergoes deimination of some arginine residues (citrullination).
Similarity: Belongs to the intermediate filament family.
Subunit Structure: Heterotetramer of two type I and two type II keratins. Heterodimer with KRT10 (PubMed:27595935). Two heterodimers of KRT1 and KRT10 form a heterotetramer (PubMed:27595935). Interacts with ITGB1 in the presence of RACK1 and SRC, and with RACK1 (PubMed:17956333). Interacts with C1QBP; the association represents a cell surface kininogen receptor (PubMed:21544310). Interacts with EPPK1; interaction is dependent of higher-order structure of intermediate filament (PubMed:16923132).
Post-translational Modifications: Undergoes deimination of some arginine residues (citrullination).
Similarity: Belongs to the intermediate filament family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67 kDa
NCBI Official Full Name
keratin, type II cytoskeletal 1
NCBI Official Synonym Full Names
keratin 1
NCBI Official Symbol
KRT1
NCBI Official Synonym Symbols
K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK
NCBI Protein Information
keratin, type II cytoskeletal 1
UniProt Protein Name
Keratin, type II cytoskeletal 1
UniProt Gene Name
KRT1
UniProt Synonym Gene Names
KRTA; CK-1; K1
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Product Notes
The K2C1 krt1 (Catalog #AAA323484) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The K2C1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's K2C1 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the K2C1 krt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "K2C1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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