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IHC (Immunohistochemistry)
(Anti-KAP1 Picoband antibody, AAA46262, IHC(P)IHC(P): Human Mammary Cancer Tissue)
KAP1 Polyclonal Antibody | anti-KAP1 antibody
Anti-KAP1 Antibody
Gene Names
TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
KAP1, Antibody; Anti-KAP1 Antibody; Transcription intermediary factor 1-beta; E3 SUMO protein ligase TRIM28; E3 SUMO-protein ligase TRIM28; FLJ29029; KAP 1; KAP-1; KRAB associated protein 1; KRAB interacting protein 1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP 1; KRIP-1; KRIP1; Nuclear corepressor KAP 1; Nuclear corepressor KAP-1; RING finger protein 96; RNF96; TF1B; TIF1 beta; TIF1-beta; TIF1B; TIF1B_HUMAN; Transcription intermediary factor 1 beta; Trim28; Tripartite motif containing 28; tripartite motif containing protein 28; Tripartite motif-containing protein 28; tripartite motif containing 28; anti-KAP1 antibody
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
835
Applicable Applications for anti-KAP1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived human KAP1 recombinant protein (Position: A699-P835). Human KAP1 shares 94.9% amino acid (aa) sequence identity with both mouse and rat KAP1.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemistry)
(Anti-KAP1 Picoband antibody, AAA46262, IHC(P)IHC(P): Human Mammary Cancer Tissue)
IHC (Immunohistochemistry)
(Anti-KAP1 Picoband antibody, AAA46262, IHC(P)IHC(P): Human Mammary Cancer Tissue)
IHC (Immunohistochemisry)
(Anti-KAP1 Picoband antibody, AAA46262, IHC(P)IHC(P): Rat Intestine Tissue)
IHC (Immunohistochemisry)
(Anti-KAP1 Picoband antibody, AAA46262, IHC(P)IHC(P): Rat Intestine Tissue)
IHC (Immunohiostchemistry)
(Anti-KAP1 Picoband antibody, AAA46262, IHC(P)IHC(P): Mouse Intestine Tissue)
IHC (Immunohiostchemistry)
(Anti-KAP1 Picoband antibody, AAA46262, IHC(P)IHC(P): Mouse Intestine Tissue)
WB (Western Blot)
(Anti-KAP1 Picoband antibody, AAA46262, Western blottingAll lanes: Anti KAP1 (AAA46262) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Rat Ovary Tissue Lysate at 50ugLane 3: SKOV Whole Cell Lysate at 40ugLane 4: COLO320 Whole Cell Lysate at 40ugLane 5: HELA Whole Cell Lysate at 40ugPredicted bind size: 105KDObserved bind size: 105KD)
WB (Western Blot)
(Anti-KAP1 Picoband antibody, AAA46262, Western blottingAll lanes: Anti KAP1 (AAA46262) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Rat Ovary Tissue Lysate at 50ugLane 3: SKOV Whole Cell Lysate at 40ugLane 4: COLO320 Whole Cell Lysate at 40ugLane 5: HELA Whole Cell Lysate at 40ugPredicted bind size: 105KDObserved bind size: 105KD)
Related Product Information for anti-KAP1 antibody
Description: Rabbit IgG polyclonal antibody for Transcription intermediary factor 1-beta(TRIM28) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Background: Tripartite motif-containing 28 (TRIM28), also known as transcriptional intermediary factor 1beta (TIF1beta) and KAP1 (KRAB-associated protein-1), is a protein that in humans is encoded by the TRIM28 gene. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. KAP1 is a ubiquitously expressed protein involved in many critical functions including: transcriptional regulation, cellular differentiation and proliferation, DNA damage repair, viral suppression, and apoptosis. Its functionality is dependent upon post-translational modifications. Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator.
Background: Tripartite motif-containing 28 (TRIM28), also known as transcriptional intermediary factor 1beta (TIF1beta) and KAP1 (KRAB-associated protein-1), is a protein that in humans is encoded by the TRIM28 gene. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. KAP1 is a ubiquitously expressed protein involved in many critical functions including: transcriptional regulation, cellular differentiation and proliferation, DNA damage repair, viral suppression, and apoptosis. Its functionality is dependent upon post-translational modifications. Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator.
References
1. Capili AD, Schultz DC, RauscherIII FJ, Borden KL (Jan 2001). "Solution structure of the PHD domain from the KAP-1 corepressor: structural determinants for PHD, RING and LIM zinc-binding domains". The EMBO Journal 20 (1-2): 165-77. 2. Iyengar, Sushma; Farnham, Peggy (2011-07-29). "KAP1 Protein: An Enigmatic Master Regulator of the Genome". The Journal of Biological Chemistry 286. 3. Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci S, Pelicci PG, Ballabio A (May 2001). "The tripartite motif family identifies cell compartments". The EMBO Journal 20 (9): 2140-51.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
79,474 Da
NCBI Official Full Name
transcription intermediary factor 1-beta
NCBI Official Synonym Full Names
tripartite motif containing 28
NCBI Official Symbol
TRIM28
NCBI Official Synonym Symbols
KAP1; TF1B; RNF96; TIF1B; PPP1R157
NCBI Protein Information
transcription intermediary factor 1-beta
UniProt Protein Name
Transcription intermediary factor 1-beta
UniProt Gene Name
TRIM28
UniProt Synonym Gene Names
KAP1; RNF96; TIF1B; TIF1-beta; KAP-1; KRIP-1
UniProt Entry Name
TIF1B_HUMAN
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Product Notes
The KAP1 trim28 (Catalog #AAA46262) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-KAP1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KAP1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the KAP1 trim28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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