WB (Western Blot)
(Western blot analysis of extracts from rat brain, using KAP2 Antibody.)
Rabbit anti-Human, Rat KAP2 Polyclonal Antibody | anti-KAP2 antibody
KAP2 Antibody
Gene Names
PRKAR2A; PKR2; PRKAR2
Reactivity
Human, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
KAP2, Antibody; KAP2 Antibody; cAMP dependent protein kinase regulatory subunit alpha 2; cAMP dependent protein kinase regulatory subunit RII alpha; cAMP dependent protein kinase type II alpha regulatory chain; cAMP dependent protein kinase type II alpha regulatory subunit; cAMP-dependent protein kinase type II-alpha regulatory subunit; KAP2; KAP2_HUMAN; MGC3606; PKR 2; PKR2; PRKA R2; PRKAR 2; PRKAR2; PRKAR2A; Protein kinase A RII alpha subunit; Protein kinase cAMP dependent regulatory type II alpha; anti-KAP2 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
KAP2 antibody detects endogenous levels of total KAP2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
404
Applicable Applications for anti-KAP2 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm. Cell Membrane. Colocalizes with PJA2 in the cytoplasm and the cell membrane.
Tissue Specificity
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Predicted Cross Reactivity
Dog
Similarity
Dog (89%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from rat brain, using KAP2 Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from rat brain, using KAP2 Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from 293 cells using KAP2 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from 293 cells using KAP2 antibody.)
IF (Immunofluorescence)
(AAA323801 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA323801 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-KAP2 antibody
Function: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Subunit Structure: The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3. Interacts with the phosphorylated form of PJA2. Interacts with MYRIP. This interaction may link PKA to components of the exocytosis machinery, thus facilitating exocytosis, including insulin release (By similarity). Forms a complex composed of PRKAR2A, GSK3B and GSKIP through GSKIP interaction; facilitates PKA-induced phosphorylation and regulates GSK3B activity (PubMed:27484798).
Post-translational Modifications: Phosphorylated by the activated catalytic chain.
Similarity: Belongs to the cAMP-dependent kinase regulatory chain family.
Subunit Structure: The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3. Interacts with the phosphorylated form of PJA2. Interacts with MYRIP. This interaction may link PKA to components of the exocytosis machinery, thus facilitating exocytosis, including insulin release (By similarity). Forms a complex composed of PRKAR2A, GSK3B and GSKIP through GSKIP interaction; facilitates PKA-induced phosphorylation and regulates GSK3B activity (PubMed:27484798).
Post-translational Modifications: Phosphorylated by the activated catalytic chain.
Similarity: Belongs to the cAMP-dependent kinase regulatory chain family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 45 kD
Predicted: 46 kDa
Predicted: 46 kDa
NCBI Official Full Name
cAMP-dependent protein kinase type II-alpha regulatory subunit isoform a
NCBI Official Synonym Full Names
protein kinase cAMP-dependent type II regulatory subunit alpha
NCBI Official Symbol
PRKAR2A
NCBI Official Synonym Symbols
PKR2; PRKAR2
NCBI Protein Information
cAMP-dependent protein kinase type II-alpha regulatory subunit
UniProt Protein Name
cAMP-dependent protein kinase type II-alpha regulatory subunit
UniProt Gene Name
PRKAR2A
UniProt Synonym Gene Names
PKR2; PRKAR2
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Product Notes
The KAP2 prkar2a (Catalog #AAA323801) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KAP2 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KAP2 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the KAP2 prkar2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KAP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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