WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A:HeLa S3; B:Hep G2; C:MOLT4) using a 10 % SDS PAGE gel and Karyopherin alpha 2 antibody at a dilution of 1:500)
Rabbit Karyopherin alpha 2 Polyclonal Antibody | anti-KPNA2 antibody
Karyopherin alpha 2 antibody
Gene Names
KPNA2; QIP2; RCH1; IPOA1; SRP1alpha
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Karyopherin alpha 2 antibody was purified by antigen-affinity chromatography
Synonyms
Karyopherin alpha 2, Antibody; Karyopherin alpha 2 antibody; Polyclonal Karyopherin alpha 2; Anti-Karyopherin alpha 2; QIP2; Karyopherin alpha -2; RAG cohort 1; IPOA1; pendulin; KPNA2; SRP1alpha; importin-alpha-P1; karyopherin subunit alpha-2; RAG cohort protein 1; Karyopherin alpha 2; SRP1-alpha; RCH1; importin alpha 1; importin subunit alpha-2; anti-KPNA2 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Karyopherin alpha 2 antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.71 mg/ml (varies by lot)
Sequence Length
529
Applicable Applications for anti-KPNA2 antibody
WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence)
Biological Significance
The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins.
Cross-Reactivity
Human
Immunogen
Karyopherin alpha 2 antibody was raised in rabbit using a synthetic peptide corresponding to a region within amino acids 468 and 529 of karyopherin alpha 2
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A:HeLa S3; B:Hep G2; C:MOLT4) using a 10 % SDS PAGE gel and Karyopherin alpha 2 antibody at a dilution of 1:500)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A:HeLa S3; B:Hep G2; C:MOLT4) using a 10 % SDS PAGE gel and Karyopherin alpha 2 antibody at a dilution of 1:500)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using karyopherin alpha 2 antibody at 1:200 dilution.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using karyopherin alpha 2 antibody at 1:200 dilution.)
Related Product Information for anti-KPNA2 antibody
Affinity purified Rabbit polyclonal Karyopherin alpha 2 antibody
Product Categories/Family for anti-KPNA2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
58 kDa
NCBI Official Full Name
karyopherin alpha 2
NCBI Official Synonym Full Names
karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
NCBI Official Symbol
KPNA2
NCBI Official Synonym Symbols
QIP2; RCH1; IPOA1; SRP1alpha
NCBI Protein Information
importin subunit alpha-1
UniProt Protein Name
Importin subunit alpha-1
UniProt Gene Name
KPNA2
UniProt Synonym Gene Names
RCH1; SRP1
UniProt Entry Name
IMA1_HUMAN
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Product Notes
The KPNA2 kpna2 (Catalog #AAA107603) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Karyopherin alpha 2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the KPNA2 kpna2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Karyopherin alpha 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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