Rabbit KBTBD5 Polyclonal Antibody | anti-KLHL40 antibody

KBTBD5 Antibody (Center)

Cat. Num. AAA290277

• Gene Names: KLHL40; NEM8; SRYP; SYRP; KBTBD5
• Reactivity: Human (Predicted Reactivity: Mouse)
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: KBTBD5, Antibody; KBTBD5 Antibody (Center); Kelch-like protein 40; Kelch repeat and BTB domain-containing protein 5; Sarcosynapsin; KLHL40; KBTBD5; SRYP; anti-KLHL40 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This KBTBD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 349-377 amino acids from the Central region of human KBTBD5.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 349-377
• Sequence Length: 621

• Applicable Applications for anti-KLHL40 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a key regulator of skeletal muscle development (PubMed:23746549). The BCR(KLHL40) complex acts by mediating ubiquitination and degradation of TFDP1, thereby regulating the activity of the E2F:DP transcription factor complex (By similarity). Promotes stabilization of LMOD3 by acting as a negative regulator of LMOD3 ubiquitination; the molecular process by which it negatively regulates ubiquitination of LMOD3 is however unclear (By similarity).
• Cellular Location: Cytoplasm {ECO:0000250|UniProtKB:Q9D783}. Cytoplasm, myofibril, sarcomere, A band Cytoplasm, myofibril, sarcomere, I band {ECO:0000250|UniProtKB:Q9D783}
• Tissue Location: Highly expressed in fetal (19, 23 and 31 weeks of gestation) and adult skeletal muscle; expression levels tend to be higher in fetal compared to postnatal muscles (at protein level). Also expressed in fetal and adult heart.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(KBTBD5 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(KBTBD5 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KBTBD5 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(KBTBD5 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the KBTBD5 antibody detected the KBTBD5 protein (arrow).)

References

Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004) :

NCBI and Uniprot Product Information

• NCBI GI #: 45593142
• NCBI GeneID: 131377
• NCBI Accession #: NP_689606.2
• NCBI GenBank Nucleotide #: NM_152393.3
• UniProt Accession #: Q2TBA0; Q86SI1; Q96MR2
• NCBI Official Full Name: kelch-like protein 40
• NCBI Official Synonym Full Names: kelch-like family member 40
• NCBI Official Symbol: KLHL40
• NCBI Official Synonym Symbols: NEM8; SRYP; SYRP; KBTBD5
• NCBI Protein Information: kelch-like protein 40
• UniProt Protein Name: Kelch-like protein 40
• UniProt Gene Name: KLHL40
• UniProt Synonym Gene Names: KBTBD5; SRYP
• UniProt Entry Name: KLH40_HUMAN