IF (Immunofluorescence)
(AAA329125 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329125 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit KC1AL Polyclonal Antibody | anti-CSNK1A1L antibody
KC1AL Antibody
Gene Names
CSNK1A1L; CK1
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
KC1AL, Antibody; KC1AL Antibody; Casein kinase 1; alpha 1 like; casein kinase 1; alpha 1-like; Casein kinase I alpha S like; Casein kinase I isoform alpha like; CK1; CKI alpha like; MGC33182; OTTHUMP00000018267; RP11 532O21.2; anti-CSNK1A1L antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
KC1AL Antibody detects endogenous levels of total KC1AL.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
337
Applicable Applications for anti-CSNK1A1L antibody
ELISA
Immunogen
A synthesized peptide derived from human KC1AL, corresponding to a region within C-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA329125 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329125 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA329125 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329125 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using KC1AL Antibody.Lane 1: C6 cells, treated with blocking peptide;Lane 2: C6 cells;Lane 3: RAW264.7 cells.Observed bands: 33 kDa.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using KC1AL Antibody.Lane 1: C6 cells, treated with blocking peptide;Lane 2: C6 cells;Lane 3: RAW264.7 cells.Observed bands: 33 kDa.)
WB (Western Blot)
(Western blot analysis of extracts from A549 c, using KC1AL Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from A549 c, using KC1AL Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-CSNK1A1L antibody
Function: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity).
Subcellular Location: Cytoplasm.
Similarity: Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Subcellular Location: Cytoplasm.
Similarity: Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)40kD, 33kD.
Predicted Molecular Weight: (Calculated)39kDa.
Predicted Molecular Weight: (Calculated)39kDa.
NCBI Official Full Name
casein kinase I isoform alpha-like
NCBI Official Synonym Full Names
casein kinase 1 alpha 1 like
NCBI Official Symbol
CSNK1A1L
NCBI Official Synonym Symbols
CK1
NCBI Protein Information
casein kinase I isoform alpha-like
UniProt Protein Name
Casein kinase I isoform alpha-like
UniProt Gene Name
CSNK1A1L
UniProt Entry Name
KC1AL_HUMAN
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Product Notes
The CSNK1A1L csnk1a1l (Catalog #AAA329125) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KC1AL Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KC1AL can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the CSNK1A1L csnk1a1l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KC1AL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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