WB (Western Blot)
(Detection of human KCC4 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-KCC4 antibody AAA212696 (lot AAA212696-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human KCC4 Polyclonal Antibody | anti-KCC4 antibody
Rabbit anti-KCC4 Antibody, Affinity Purified
Gene Names
SLC12A7; KCC4
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
KCC4, Antibody; Rabbit anti-KCC4 Antibody, Affinity Purified; solute carrier family 12 (potassium/chloride transporters), member 7; solute carrier family 12 member 7; solute carrier family 12 (potassium/chloride transporter), member 7; potassium/chloride transporter KCC4; K-Cl cotransporter 4; KCC4; electroneutral potassium-chloride cotransporter 4; SUCO/SLC12A7 fusion; Solute carrier family 12 member 7; anti-KCC4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-KCC4 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rabbit
Immunogen
Between 950 and 1000
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human KCC4 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-KCC4 antibody AAA212696 (lot AAA212696-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human KCC4 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-KCC4 antibody AAA212696 (lot AAA212696-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human KCC4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-KCC4 antibody AAA212696 (lot AAA212696-1) used for IP at 6 ug per reaction. KCC4 was also immunoprecipitated by rabbit anti-KCC4 antibody For blotting immunoprecipitated KCC4, AAA212696 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human KCC4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-KCC4 antibody AAA212696 (lot AAA212696-1) used for IP at 6 ug per reaction. KCC4 was also immunoprecipitated by rabbit anti-KCC4 antibody For blotting immunoprecipitated KCC4, AAA212696 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-KCC4 antibody
K-Cl cotransporter 4 (KCC4) mediates electroneutral potassium-chloride cotransport when activated by cell swelling. KCC4 may mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. KCC4 is important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. It may be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y666].
Product Categories/Family for anti-KCC4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,258 Da
NCBI Official Full Name
solute carrier family 12 member 7
NCBI Official Synonym Full Names
solute carrier family 12 member 7
NCBI Official Symbol
SLC12A7
NCBI Official Synonym Symbols
KCC4
NCBI Protein Information
solute carrier family 12 member 7
UniProt Protein Name
Solute carrier family 12 member 7
UniProt Gene Name
SLC12A7
UniProt Synonym Gene Names
KCC4
UniProt Entry Name
S12A7_HUMAN
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Product Notes
The KCC4 slc12a7 (Catalog #AAA212696) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-KCC4 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KCC4 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the KCC4 slc12a7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KCC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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