WB (Western Blot)
(All lanes : Anti-KCNH1 Antibody (C-Term) at 1:2000 dilutionLane 1: COLO 205 whole cell lysateLane 2: Human brain lysateLane 3: Hela whole cell lysateLane 4: MCF-7 whole cell lysateLane 5: SH-SY5Y whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 111 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit KCNH1 Polyclonal Antibody | anti-KCNH1 antibody
KCNH1 Antibody (C-Term)
Gene Names
KCNH1; EAG; EAG1; ZLS1; TMBTS; h-eag; hEAG1; Kv10.1
Reactivity
HumanPredicted: Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Purified through a protein A column, followed by peptide affinity purification.
Synonyms
KCNH1, Antibody; KCNH1 Antibody (C-Term); Purified Rabbit Polyclonal Antibody (Pab); Potassium voltage-gated channel subfamily H member 1; Ether-a-go-go potassium channel 1; EAG channel 1; h-eag; hEAG1; Voltage-gated potassium channel subunit Kv10.1; KCNH1; EAG; EAG1; anti-KCNH1 antibody
Host
Rabbit
Reactivity
Human
Predicted: Human
Predicted: Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This KCNH1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 787-820 amino acids from the human region of human KCNH1.
Purity/Purification
Purified through a protein A column, followed by peptide affinity purification.
Form/Format
Supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
962
Applicable Applications for anti-KCNH1 antibody
ELISA, WB (Western Blot), FCM/FACS (Flow Cytometry)
Antigen Type
Synthesized Peptide
Antigen Source
Human
Tissue Location
Highly expressed in brain and in myoblasts at the onset of fusion, but not in other tissues. Detected in HeLa (cervical carcinoma), SH-SY5Y (neuroblastoma) and MCF-7 (epithelial tumor) cells, but not in normal epithelial cells
Cellular Location
Cell membrane; Multi-pass membrane protein. Nucleus inner membrane; Multi-pass membrane protein. Cell projection, dendrite {ECO:0000250|UniProtKB:Q63472}. Cell projection, axon {ECO:0000250|UniProtKB:Q63472}. Cell junction, synapse, presynaptic cell membrane {ECO:0000250|UniProtKB:Q63472} Perikaryon {ECO:0000250|UniProtKB:Q63472}. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density {ECO:0000250|UniProtKB:Q63472}. Early endosome membrane. Note=Perinuclear KCNH1 is located to NPC-free islands
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-KCNH1 Antibody (C-Term) at 1:2000 dilutionLane 1: COLO 205 whole cell lysateLane 2: Human brain lysateLane 3: Hela whole cell lysateLane 4: MCF-7 whole cell lysateLane 5: SH-SY5Y whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 111 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-KCNH1 Antibody (C-Term) at 1:2000 dilutionLane 1: COLO 205 whole cell lysateLane 2: Human brain lysateLane 3: Hela whole cell lysateLane 4: MCF-7 whole cell lysateLane 5: SH-SY5Y whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 111 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AP22343b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22343b, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AP22343b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22343b, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Related Product Information for anti-KCNH1 antibody
Pore-forming (alpha) subunit of a voltage-gated delayed rectifier potassium channel (PubMed:9738473, PubMed:11943152, PubMed:10880439, PubMed:22732247, PubMed:25556795, PubMed:27325704, PubMed:27005320, PubMed:27618660). Channel properties are modulated by subunit assembly (PubMed:11943152). Mediates IK(NI) current in myoblasts (PubMed:9738473). Involved in the regulation of cell proliferation and differentiation, in particular adipogenic and osteogenic differentiation in bone marrow-derived mesenchymal stem cells (MSCs) (PubMed:23881642).
Product Categories/Family for anti-KCNH1 antibody
References
Occhiodoro T.,et al.FEBS Lett. 434:177-182(1998). Pardo L.A.,et al.EMBO J. 18:5540-5547(1999). Gregory S.G.,et al.Nature 441:315-321(2006). Mural R.J.,et al. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Schoenherr R.,et al.EMBO J. 19:3263-3271(2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
111423
NCBI Official Full Name
potassium voltage-gated channel subfamily H member 1 isoform 2
NCBI Official Synonym Full Names
potassium voltage-gated channel subfamily H member 1
NCBI Official Symbol
KCNH1
NCBI Official Synonym Symbols
EAG; EAG1; ZLS1; TMBTS; h-eag; hEAG1; Kv10.1
NCBI Protein Information
potassium voltage-gated channel subfamily H member 1
UniProt Protein Name
Potassium voltage-gated channel subfamily H member 1
UniProt Gene Name
KCNH1
UniProt Synonym Gene Names
; h-eag; hEAG1
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Product Notes
The KCNH1 kcnh1 (Catalog #AAA290906) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KCNH1 Antibody (C-Term) reacts with Human Predicted: Human and may cross-react with other species as described in the data sheet. AAA Biotech's KCNH1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the KCNH1 kcnh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KCNH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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