Rabbit KCNH2 / HERG Polyclonal Antibody | anti-KCNH2 antibody

Rabbit Polyclonal to Human KCNH2 / HERG

Cat. Num. AAA52076

• Gene Names: KCNH2; ERG1; HERG; LQT2; SQT1; ERG-1; H-ERG; HERG1; Kv11.1
• Reactivity: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit; Predicted: Bat, Guinea pig
• Applications: Immunohistochemistry, Immunohistochemistry
• Purity: Immunoaffinity purified
• Synonyms: KCNH2 / HERG, Antibody; Rabbit Polyclonal to Human KCNH2 / HERG; Anti-KCNH2 / HERG Antibody (Internal) IHC-plus; KCNH2; Eag homolog; HERG-1; ERG1; HERG; KV11.1; M-erg1; H-ERG; HERG1; SQT1; LQT2; Merg1; Eag-related protein 1; ERG-1; Human KCNH2; anti-KCNH2 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit; Predicted: Bat, Guinea pig
• Clonality: Polyclonal
• Specificity: Human KCNH2 / Kv11.1. BLAST analysis of the peptide immunogen showed no homolgy with other human proteins.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, 0.1% Sodium Azide
• Concentration: 0.9 mg/ml (varies by lot)

• Applicable Applications for anti-KCNH2 antibody: IHC (Immunohistochemistry), IHC (Immunohistochemistry)
• Target: KCNH2 / HERG
• Family / Subfamily: Ion Channel / Potassium channel - Eag-related
• Host Note: KCNH2 / HERG antibody was produced in Rabbit
• Immunogen Species: KCNH2 / HERG antibody was raised against Human
• Antigen Type: Synthetic peptide
• Immunogen: KCNH2 / HERG antibody was raised against synthetic 16 amino acid peptide from internal region of human KCNH2 / Kv11.1. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Pig, (100%); Bat, Guinea pig (94%)
• Epitope: Internal
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Product Categories/Family for anti-KCNH2 antibody

Family: Ion Channel; Subfamily: Potassium channel - Eag-related

NCBI and Uniprot Product Information

• NCBI GI #: 4557729
• NCBI GeneID: 3757
• NCBI Accession #: NP_000229.1
• NCBI GenBank Nucleotide #: NM_000238.3
• UniProt Accession #: Q12809; O75418; O75680; Q708S9; Q9BT72; Q9BUT7; Q9H3P0; A5H1P7; C4PFH9; D3DX04
• NCBI Official Full Name: potassium voltage-gated channel subfamily H member 2 isoform a
• NCBI Official Synonym Full Names: potassium voltage-gated channel, subfamily H (eag-related), member 2
• NCBI Official Symbol: KCNH2
• NCBI Official Synonym Symbols: ERG1; HERG; LQT2; SQT1; ERG-1; H-ERG; HERG1; Kv11.1
• NCBI Protein Information: potassium voltage-gated channel subfamily H member 2; eag homolog; eag-related protein 1; ether-a-go-go-related protein 1; ether-a-go-go-related gene potassium channel 1; voltage-gated potassium channel subunit Kv11.1; ether-a-go-go-related potassium channel protein
• UniProt Protein Name: Potassium voltage-gated channel subfamily H member 2
• UniProt Gene Name: KCNH2
• UniProt Synonym Gene Names: ERG; ERG1; HERG; ERG-1; Eag-related protein 1; Ether-a-go-go-related protein 1; H-ERG; hERG-1; hERG1
• UniProt Entry Name: KCNH2_HUMAN