IF (Immunofluorescence)
(AAA327883 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit KCNMB1 Polyclonal Antibody | anti-KCNMB1 antibody
KCNMB1 Antibody
Gene Names
KCNMB1; hbeta1; BKbeta1; SLO-BETA; hslo-beta; K(VCA)beta; slo-beta-1; k(VCA)beta-1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
KCNMB1, Antibody; KCNMB1 Antibody; BK channel beta subunit; BK channel subunit beta 1; BKbeta; BKbeta1; Calcium activated potassium channel subfamily M subunit beta 1; Calcium activated potassium channel subunit beta 1; Calcium activated potassium channel subunit beta; Charybdotoxin receptor subunit beta 1; Hbeta1; hslo beta; K(VCA)beta 1; K(VCA)beta; KCNMB 1; KCNMB1; Large conductance Ca2+ activated K+ channel beta 1 subunit; Maxi K channel beta subunit; Maxi K channel subunit beta 1; Potassium large conductance calcium activated channel subfamily M beta member 1; Slo beta 1; SLO beta; anti-KCNMB1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
KCNMB1 antibody detects endogenous levels of total KCNMB1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
191
Applicable Applications for anti-KCNMB1 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human KCNMB1
Subcellular Location
Membrane; Multipass Membrane Protein
Tissue Specificity
Abundantly expressed in smooth muscle. Low levels of expression in most other tissues. Within the brain, relatively high levels found in hippocampus and corpus callosum.
Predicted Cross Reactivity
Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Bovine (90%), Horse (100%), Sheep (90%), Rabbit (80%), Dog (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA327883 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA327883 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
WB (Western Blot)
(Western blot analysis of KCNMB1 using COS7 whole cell lysates)
WB (Western Blot)
(Western blot analysis of KCNMB1 using COS7 whole cell lysates)
WB (Western Blot)
(Western blot analysis of extracts from rat brain, using KCNMB1 Antibody. Lane 1 was treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of extracts from rat brain, using KCNMB1 Antibody. Lane 1 was treated with the antigen-specific peptide.)
Related Product Information for anti-KCNMB1 antibody
Function: Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Increases the apparent Ca2+/voltage sensitivity of the KCNMA1 channel. It also modifies KCNMA1 channel kinetics and alters its pharmacological properties. It slows down the activation and the deactivation kinetics of the channel. Acts as a negative regulator of smooth muscle contraction by enhancing the calcium sensitivity to KCNMA1. Its presence is also a requirement for internal binding of the KCNMA1 channel opener dehydrosoyasaponin I (DHS-1) triterpene glycoside and for external binding of the agonist hormone 17-beta-estradiol (E2). Increases the binding activity of charybdotoxin (CTX) toxin to KCNMA1 peptide blocker by increasing the CTX association rate and decreasing the dissociation rate.
Subunit Structure: Interacts with KCNMA1 tetramer. There are probably 4 molecules of KCMNB1 per KCNMA1 tetramer.
Post-translational Modifications: N-glycosylated.
Similarity: Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB1 subfamily.
Subunit Structure: Interacts with KCNMA1 tetramer. There are probably 4 molecules of KCMNB1 per KCNMA1 tetramer.
Post-translational Modifications: N-glycosylated.
Similarity: Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB1 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 22 kDa
Predicted: 22 kDa
Predicted: 22 kDa
NCBI Official Full Name
calcium-activated potassium channel subunit beta-1
NCBI Official Synonym Full Names
potassium calcium-activated channel subfamily M regulatory beta subunit 1
NCBI Official Symbol
KCNMB1
NCBI Official Synonym Symbols
hbeta1; BKbeta1; SLO-BETA; hslo-beta; K(VCA)beta; slo-beta-1; k(VCA)beta-1
NCBI Protein Information
calcium-activated potassium channel subunit beta-1
UniProt Protein Name
Calcium-activated potassium channel subunit beta-1
UniProt Gene Name
KCNMB1
UniProt Synonym Gene Names
BKbeta; BKbeta1; Hbeta1; Calcium-activated potassium channel subunit beta; Slo-beta
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Product Notes
The KCNMB1 kcnmb1 (Catalog #AAA327883) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KCNMB1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KCNMB1 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the KCNMB1 kcnmb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KCNMB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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