Rabbit anti-Human, mouse KCTD21 Polyclonal Antibody | anti-KCTD21 antibody

KCTD21 Antibody (C-term)

Cat. Num. AAA286750

• Gene Names: KCTD21; KCASH2
• Reactivity: Human, mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: KCTD21, Antibody; KCTD21 Antibody (C-term); BTB/POZ domain-containing protein KCTD21; KCTD21; anti-KCTD21 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This KCTD21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232-260 amino acids from the C-terminal region of human KCTD21.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 232-260
• Sequence Length: 260

• Applicable Applications for anti-KCTD21 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Probable substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex mediating the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes the ubiquitination of HDAC1. Can function as antagonist of the Hedgehog pathway by affecting the nuclear transfer of transcription factor GLI1; the function probably occurs via HDAC1 down-regulation, keeping GLI1 acetylated and inactive. Inhibits cell growth and tumorigenicity of medulloblastoma (MDB) (PubMed:21472142).
• Tissue Location: Highly expressed in cerebellum and brain. Expression is down-regulated in medulloblastoma.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(KCTD21 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(KCTD21 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KCTD21 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(KCTD21 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the KCTD21 antibody detected the KCTD21 protein (arrow).)

Product Categories/Family for anti-KCTD21 antibody

Cell biology; Neuroscience

References

Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004) :

NCBI and Uniprot Product Information

• NCBI GI #: 71143104
• NCBI GeneID: 283219
• NCBI Accession #: NP_001025030.1
• NCBI GenBank Nucleotide #: NM_001029859.2
• UniProt Accession #: Q4G0X4; B4DTR0
• Molecular Weight: 29643
• NCBI Official Full Name: BTB/POZ domain-containing protein KCTD21
• NCBI Official Synonym Full Names: potassium channel tetramerization domain containing 21
• NCBI Official Symbol: KCTD21
• NCBI Official Synonym Symbols: KCASH2
• NCBI Protein Information: BTB/POZ domain-containing protein KCTD21
• UniProt Protein Name: BTB/POZ domain-containing protein KCTD21
• UniProt Gene Name: KCTD21
• UniProt Entry Name: KCD21_HUMAN