IF (Immunofluorescence)
(AAA328916 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328916 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit KDM6A/UTX Polyclonal Antibody | anti-KDM6A antibody
KDM6A/UTX Antibody
Gene Names
KDM6A; UTX; KABUK2; bA386N14.2
Reactivity
Human, Mouse, RatPredicted Reactivity: Zebrafish(83%), Bovine(100%), Sheep(100%), Rabbit(88%), Dog(100%), Chicken(100%), Xenopus(88%)
Applications
ELISA, Immunocytochemistry, Immunofluorescence
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
KDM6A/UTX, Antibody; KDM6A/UTX Antibody; bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX); bA386N14.2; bA386N14.2 ubiquitously transcribed X chromosome tetratricopeptide repeat protein UTX; DKFZp686A03225; Histone demethylase UTX; KABUK2; Kdm6a; KDM6A_HUMAN; Lysine (K) specific demethylase 6A; Lysine K specific demethylase 6A; Lysine specific demethylase 6A ubiquitously transcribed tetratricopeptide repeat gene X chromosome; Lysine-specific demethylase 6A; MGC141941; Ubiquitously transcribed tetratricopeptide repeat X chromosome; Ubiquitously transcribed tetratricopeptide repeat protein X linked; Ubiquitously transcribed TPR gene on the X chromosome; Ubiquitously transcribed TPR protein on the X chromosome; Ubiquitously transcribed X chromosome tetratricopeptide repeat protein; Ubiquitously-transcribed TPR protein on the X chromosome; Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein; UTX; anti-KDM6A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Zebrafish(83%), Bovine(100%), Sheep(100%), Rabbit(88%), Dog(100%), Chicken(100%), Xenopus(88%)
Predicted Reactivity: Zebrafish(83%), Bovine(100%), Sheep(100%), Rabbit(88%), Dog(100%), Chicken(100%), Xenopus(88%)
Clonality
Polyclonal
Isotype
IgG
Specificity
KDM6A / UTX Antibody detects endogenous levels of total KDM6A / UTX.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
1401
Applicable Applications for anti-KDM6A antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
A synthesized peptide derived from human KDM6A / UTX, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA328916 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328916 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA328916 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328916 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
WB (Western Blot)
(Western blot analysis of extracts from MCF7, using KDM6A / UTX Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from MCF7, using KDM6A / UTX Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-KDM6A antibody
Function: Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed:17851529, PubMed:17713478, PubMed:17761849). Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27' (PubMed:17851529, PubMed:17713478, PubMed:17761849). Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed:17851529). Demethylation of 'Lys-27' of histone H3 is concomitant with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A (PubMed:17761849). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression (By similarity).
Subcellular Location: Nucleus.
Subunit Structure: Interacts with TLE1 (By similarity). Component of the MLL2/3 complex (also named ASCOM complex), at least composed of KMT2D/MLL2 or KMT2C/MLL3, ASH2L, RBBP5, WDR5, NCOA6, DPY30, KDM6A (or KDM6B), PAXIP1/PTIP, PAGR1 and alpha- and beta-tubulin (PubMed:17500065, PubMed:17713478). Interacts with SUPT6H. Interacts with SMARCA4 (By similarity).
Similarity: Belongs to the UTX family.
Subcellular Location: Nucleus.
Subunit Structure: Interacts with TLE1 (By similarity). Component of the MLL2/3 complex (also named ASCOM complex), at least composed of KMT2D/MLL2 or KMT2C/MLL3, ASH2L, RBBP5, WDR5, NCOA6, DPY30, KDM6A (or KDM6B), PAXIP1/PTIP, PAGR1 and alpha- and beta-tubulin (PubMed:17500065, PubMed:17713478). Interacts with SUPT6H. Interacts with SMARCA4 (By similarity).
Similarity: Belongs to the UTX family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)154kD.
Predicted Molecular Weight: (Calculated)154kDa.
Predicted Molecular Weight: (Calculated)154kDa.
NCBI Official Full Name
lysine-specific demethylase 6A isoform 3
NCBI Official Synonym Full Names
lysine demethylase 6A
NCBI Official Symbol
KDM6A
NCBI Official Synonym Symbols
UTX; KABUK2; bA386N14.2
NCBI Protein Information
lysine-specific demethylase 6A
UniProt Protein Name
Lysine-specific demethylase 6A
UniProt Gene Name
KDM6A
UniProt Synonym Gene Names
UTX
UniProt Entry Name
KDM6A_HUMAN
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Product Notes
The KDM6A kdm6a (Catalog #AAA328916) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KDM6A/UTX Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Zebrafish(83%), Bovine(100%), Sheep(100%), Rabbit(88%), Dog(100%), Chicken(100%), Xenopus(88%) and may cross-react with other species as described in the data sheet. AAA Biotech's KDM6A/UTX can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the KDM6A kdm6a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KDM6A/UTX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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